INVOLVENT OF ANGIOTENSIN AND RECENTLY DEORPHANISED RECEPTORS IN REGULATION OF  PULMONARY VESSELS AND BRONCHIAL  REACTIVITY ON PULMONARY HYPERTENSION AND BRONCHIAL HYPERRESPONSIVENESS EXPERIMENTAL MODELS

The orphan G proteins coupled receptors represent an immense potential for the development of growing collection of chemical tools and potential therapeutic compounds. We are the first demonstrating that both apelin (AP)13 and urotensin II (UII) could modulate the acetylcholine - induced bronhoconstriction by a nitric oxide (no)-dependent mechanism. Experimental and clinical studies illustrate no involvement in multiple physiological and pathological conditions. Hyperactivity and hypertrophy was identified on both airways on pulmonary hypertension and pulmonary vessels on asthma, suggesting that alterations are happening simultaneously on vessels and bronchi. The pulmonary renin angiotensin system (RAS) plays a key role on these pathological states. Our original studies emphasized roles of pulmonary synthesized angiotensin on airways tone. Ang II synthesized locally in airway walls could modify acetylcholine contractile effects. This project proposes study of both implications and interactions of RAS and ligand-orphan receptor systems on monocrotaline induced pulmonary hypertension and ovalbumin induced bronchial hyperreactivity. Pulmonary vessels and bronchi rings reactivity and no synthesis will be simultaneous assess with wire miograph and NO-meter. Distribution of APJ and GPR14 receptors will be estimate by immunofluorescence. Inflammatory and remodeling processes will be monitories by histology/ immunohistochemistry/ serology. In vivo measurement of airways resistance with/without RAS blockers will substantiate our results.

Links:

THE NATIONAL UNIVERSITY RESEARCH COUNCIL

THE ROMANIAN SOCIETY OF PHYSIOLOGICAL SCIENCES

UNIVERSITY OF MEDICINE AND PHARMACY "GR.T. POPA" IASI

PubMed

PROJECT No. 1273/2007-2010 CNCSIS

Name:

BOGDAN GURZU, MD, PhD

Email:

bgurzu@yahoo.com