Things to be concern in enzyme exp
Plan for variables:
Eg. The effect of substrate conc. on urease activity
Independent var:substrate conc.(urea)
Dependent var:rate of reaction
Control var:enzyme(urease) conc.
Temperature(both enzyme & sub.medium)
Volume of enzyme and reaction medium
Extract enzyme in living tissue
Soak, gring tissue.
Add KNOWN amount of water!!
Time once you mix the enzyme into the sub medium
Make the faster as end point reference