Senior Scientist (Biotechnology)
Division of Crop Improvement
Indian Grassland & Fodder Research Institute
Jhansi - 284 003 (U.P.) INDIA.
Date of Birth: 05.01.1970.
Academic Qualifications:
* B. Sc. (Agriculture & Animal Husbandry) from Chandra Shekhar Azad University of Agriculture and Technology, Kanpur, Uttar Pradesh, India, in the year 1994 with 88.56 % marks (Gold Medalist).
* M. Sc. (Biotechnology) from Tamil Nadu Agricultural University, Coimbatore, Tamil Nadu, India, in the year 1997 with 94.2 % marks.
* Ph. D. (Molecular Biology and Biotechnology) from National Research Center on Plant Biotechnology, Indian Agricultural Research Institute, New Delhi - 110 012, India.
Research Experience:
(a) Molecular Biology laboratory techniques : -
* Isolation and Purification of Genomic DNA, RNA, Proteins and Enzymes
* Bacterial transformation and Plasmid amplification
(b) Tissue Culture techniques : -
* Tissue and cell suspension culture of Japonica and Indica rice varieties
* Tissue culture regeneration of forage crops (legumes and grasses)
(c) Plant Transformation techniques : -
* Agrobacterium-mediated transformation of Japonica and Indica rice varieties
* DNA Gun (Biolistic PDS-1000/He, BioRad) mediated transformation of Japonica and Indica rice
* Particle-Inflow Gun-mediated transformation of forage crops viz. Cenchrus, and Dichanthium
* Agrobacterium-mediated transformation of forage crops viz. Cenchrus, Dichanthium and Medicago.
* Analysis of transgenic plants using PCR and Southern Hybridization
Research accomplishment :
* Developed an efficient protocol for Agrobacterium-mediated transformation of Japonica and Indica rice varieties
* Developed an efficient protocol for Biolistic-mediated transformation of Japonica and Indica rice varieties
* Regeneration protocol optimized for Cenchrus ciliaris, Dichanthium annulatum and Medicago sativa
* Agrobacterium-mediated transformation of Medicago sativa and Cenchrus ciliaris.
* Particle-Inflow Gun-mediated transformation of Cenchrus and Dichanthium
Areas of Interest :
1. Genetic manipulation of crop plants.
2. Molecualr biology of apomixis.
3. Stress biology of crop plants.
suresh.kumar3@lycos.com
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