BIOGRAPHICAL SKETCH                                             11/03

 

Xiangzhong (Jerry) Yang

 

Advanced Technology Laboratory Building                       

Center for Regenerative Biology                                                  

University of Connecticut, Unit 4243                                           

Storrs, CT 06269-4243                                                              

860-486-8728 (phone)/x-3536 (fax)

 

CURRENT POSITION:             Professor of Animal Science and Biotechnology

Director, Center for Regenerative Biology

Director, China Bridges International Fellowship Program

University of Connecticut, Storrs, CT

 

Adjunct Professor of International Agriculture

Cornell University, Ithaca, NY

 

CAREER GOAL:                      To be an outstanding researcher and teacher in the area of reproductive biology and animal biotechnology, with special emphasis on cattle, pigs and laboratory animals

EDUCATION:

 

1990     Ph.D.    Department of Animal Science, Cornell University

Major: Animal Science (Reproductive Physiology with R.H. Foote)

Minors: Veterinary Physiology and Animal Nutrition

 

Major achievements: Studied nuclear-cytoplasmic interactions by microinjection and nuclear transfer; Developed novel micromanipulation technologies for sperm injection (Yang et al., 1990a) and nuclear transfer (Yang et al., 1990b, c) and produced several offspring from cloned rabbit embryos (Yang et al., 1992).  Pioneered the procedure of using hypertonic medium to assist micromanipulation, which has been widely adapted for microinjection work both in animals and in humans.

 

1987     M.S.     Department of Animal Science, Cornell University

Major:  Animal Science (with R.H. Foote); Minor:  Animal Nutrition

 

Major achievements:  Developed novel techniques for producing identical twins (Yang and Foote, 1987) and chimeric rabbits (Yang and Foote, 1988); Established optimal embryo and uterine synchrony for transferring micromanipulated embryos (Yang and Foote, 1990).  The technique of microinjection of embryonic cells into morulae was later widely used for testing embryonic stem cells in rabbits and mice.

 

1982     B.S.      (with Honors) Animal Science, Beijing Agricultural University

Honors thesis:  Superovulation Responses in Cattle

 

EMPLOYMENT AT UNIVERSITY OF CONNECTICUT

 

2001-        Director, Center for Regenerative Biology (CRB)

2002-        Professor of Animal Science and Biotechnology

1996-     Head, Biotechnology Center Transgenic Animal Facility

1996-   Director, China Bridges International Fellowship Program, Division of International Affairs

            1996-2000       Associate Professor of Animal Science and Biotechnology

 

Major achievements:    My major achievements at UCONN are summarized bellow:

- Research:  I have successfully attracted over $8,000,000 extra-mural supports from the USDA, NIH, the State of Connecticut (CII), the Rockefeller Foundation, and the pharmaceutical industry, such as Alexion, Biotechnology Research and Development Corporation (BRDC), PPL Therapeutics and Genzyme Transgenic Corporation etc.  I have published more than 90 peer-reviewed papers (6-10 papers/year) and over 100 conference proceeding and technical papers.  In 2000-2002, two of our papers were selected as the cover paper in two prestigious journals: Biology of Reproduction (cover issues of January to June 2000) and the Proceedings of the National Academy of Sciences (PNAS, the February 1, 2000 issue) and two papers published in Nature Genetics (2000 and 2002).  Because of my scientific achievements in our field, I have been invited as a keynote, featured or seminar speaker (over 150 since 1996) at many scientific conferences or educational/research institutions around the world.  Under my leadership, the Transgenic Animal Facility has attracted several collaboration research contract grants from several international leading transgenic animal companies (e.g., PPL, Genzyme, Pharming, and Alexion) and has assisted numerous UCONN faculty members on their grant proposals.  Our recent success on cattle and pig cloning (announced in June 1999, January and November 2000, April and July 2001, March and May 2002) has been covered extensively by various news media around the world.  These publicities include The CNN Headline News, BBC News, CBS News, NPR News, The New York Times, The Washington Post, The Wall Street Journal, The US News & World Reports, The Chicago Tribune, The Boston Globe, London Times, Associated Press, Reuters, Agency France Press, China Press and Xinhua News Press etc.  My achievements in research have been widely recognized by many awards and honors, including the most recent University of Connecticut Chancellor Research Excellence Award (1999), Chancellor Special Achievements Awards (1999; 2000), The CANR Research Excellence Award (2000), and the University of Connecticut Chapter AAUP Research Excellence Award (2000).  I have also been recognized recently by an official citation to recognize my research achievements from the General Assembly (2000) and the Senate (2000) of the State of Connecticut. In 2001, I was awarded with the International Research Award on Animal Reproduction from the Society of Assisted Reproductive Technology/Andrology, NC.  Recently, I received the 2002 Science and Technology Innovation Award (from Beijing High-Tech Commission, China) and the 2003 Asian American Achievements Award (from the Asian American Heritage Council, NJ, presented by the NJ Governor).

 

-Teaching: I have developed two new courses, offered several mini-courses (workshops) and delivered many guest lectures at UCONN in the areas of reproductive biology and biotechnology.  The evaluations from students in both courses that I have taught are excellent (scoring 9.5 to 9.6 out of a possible score of 10).  In addition, I have been supervising about 8-10 undergraduate independent research projects or honors' projects each year.  Further, I have trained over 50 graduate, post-doctoral students or visiting scientists in my program. Currently, I am supervising an extramurally funded research team of 20 members including 4 postdoctoral scientists, 10 graduate students, 3 research assistants (technicians) and 2 visiting scientists.

 

-Public services: I have served on numerous departmental, college and university-wide committees, including the most recent Chancellor Search Advisory Committee, VP for Research and Graduate Education Search Committee, the CSTC Advisory Committee and the China Steering Committee.  Currently, I am the chairperson of the departmental graduate committee and coordinator of the departmental seminar series.  My public services also include frequent public speeches statewide at meetings or gatherings (visiting and touring) of UCONN alumni associations, UCONN Cooperative Extension Systems, schoolteachers, farmers, junior and senior high school students, Future Farmers Association, State of Connecticut Department of Agriculture, and Department of Education etc.  I have also served on numerous national and international committees and delivered over 100 invited scientific talks or seminars at scientific conferences or educational institutions.  In addition, I have received grants and have been directing an international fellowship program (China Bridges) with an operation budget of over $1,000,000 (grants from the Rockefeller Foundation and Lingnan Foundation) plus matching funds from the National Science Foundation of China.

 

EMPLOYMENT AT CORNELL:

 

91-96        Senior Research Associate-I and Program Director (Independent PI)

My responsibility was to develop an independent and extramurally funded research program (laboratory) in the Department of Animal Science at Cornell. My primary areas of research were to investigate the mechanisms of oocyte maturation, fertilization, early embryo development and cloning.  Between 1991 and 1996, I prepared several dozens of grant proposals as PI or co-PI and received approximately $2,700,000 of external grant supports. I have trained 12 post-doctoral or visiting scientists, two Ph.D. students and over 20 undergraduate students for their research or honors thesis projects.

 

90-91        Postdoctoral Associate in Embryo Biotechnology and Reproductive Biology.

During my 2-year (20 months) post-doctoral training, I studied the mechanisms and control of oocyte maturation, IVF, activation, early embryonic development and cloning in cattle following various genetic manipulations.  During this period, I have succeeded the production of cloned rabbits and cattle using embryonic cells as nuclear donors.  Selected publications from my post-doctoral training include: Yang, 1991, 1992; Yang and Anderson, 1992; Foote and Yang, 1992; Yang et al., 1992, 1993a, b; Ellington et al., 1993; Giles et al., 1993; and Heuwieser et al., 1992a, b.

 

HONORS/AWARDS:

      a).  Awards:

2003     Asian American Achievements Award (from the Asian American Heritage Council, NJ, presented by the NJ Governor).

2002          Science and Technology Innovation Award (from Beijing High-Tech Commission, China)

2001     International ARTA Award, Society of Assisted Reproductive Technology/Andrology, NC

2000          AAUP Research Excellence Award, the University of Connecticut Chapter of the American Association of University Professors

2000     Official Citation for the Recognition of Research Excellence by the General Assembly of the State of Connecticut

2000     Official Citation for the Recognition of Research Excellence by the State of Connecticut Senate

2000     CANR Research Excellence Award, The University of Connecticut

1999          Chancellor's Special Achievement Award, The University of Connecticut

1999     Dean's Special Achievement Merit Award, The University of Connecticut

1999     Chancellor Research Excellence Award, The University of Connecticut

1999     Banquet Honor to celebrate Jerry Yang's success on cattle cloning by the Chinese Ambassador to the United States

1994     First Runner-up Award of the Graduate Student Research Competitions (Advisor to Bin Wu, Graduate Student), International Embryo Transfer Society (IETS)

1992     Winner of Graduate Student Research Competitions (Advisor to Lynne Moraghan, Undergraduate Researcher), International Embryo Transfer Society (IETS)

1985     Fellowship, the Cornell University Graduate School

83-85    Fellowship for Overseas Studies, the State Education Commission, P.R. China

1981     Winner (First Place), The National Entrance Examination for Graduate Studies, Beijing Agricultural University, P.R. China

 

      b).  Honorary Appointment (1991 to present):

Adjunct Professor of International Agriculture, Cornell University

Honorary Professor, Xinjiang Academy of Sciences

Honorary Professor, China Agricultural University

Honorary Professor, The Northwest Agricultural University

Honorary Professor The Chinese Academy of Agricultural Sciences

Honorary Professor, The Chinese Academy of Sciences

Science and Technology Advisor to the Chinese Ministry of Agriculture

Elected Member of the External Review Council for the Chinese Academy of Sciences

External Advisor, Yangling National Demonstration Zone of Agricultural Hi-Tech Industry

Honorary President, Handan City International Friendship Association

Honorary President, Bazhong Education Promotion Association (Sichuan)

Honorary Professor, Shanghai Academy of Agricultural Sciences, Shanghai

Honorary Professor, Shandong Academy of Agricultural Sciences, Jinan, Shandong

Honorary Professor, Yangzhou University, Yangzhou, Jiangsu

 

c). Invited conference lectures/Keynote speech (since 1996):

 

1996

Special lecture, the Third Congress of the Japanese Embryo Transfer Society

 

1997

Keynote speaker, 9th Brazilian Embryo Transfer Society Annual Meeting

Expert panelist, New York State Senate Hearing on Cloning

 

1998

Invited speaker, 14th International Embryo Transfer Society Annual Meeting

Conference chairperson and keynote speaker, International Conference on “Transgenic Production of Human Therapeutics”

Keynote speaker, the 1998 National Italian Agricultural Biotechnology Symposium

Invited experts panelist, DOC Advanced Technology Program Electronic Workshop

Keynote speaker, Sino-US Joint Conference on Biotechnology and Medicine in 21st Century. Beijing, China

 

1999

Symposium Co-organizer and Keynote Speaker, Sino-US Agricultural Biotechnology Symposium, Xi'an

Keynote speaker and plenary session Chair, International Symposium on the 21st Century China.  Washington DC.

Invited speaker, 2nd International Transgenic Animal Research Conference.  Lake Tahoe, CA.

Featured speaker, 7th Annual Conference of Sino-American Pharmaceutical Association.  New Brunswick

 

2000

Invited speaker, Banbury Conference on Mammalian Cloning: Biology and Practice. Cold Spring Harbor, NY

Keynote speaker, International Symposium on Managing Bio-Resources and Bio-Diversity: Agriculture of the New Century.  Taiwan

Invited speaker, Symposium on Bioengineering of Farm Animals: Legal and Ethical Issues.  New York, NY

Invited speaker, Symposium on Genetically Engineering and Cloning Animals.  Park City, UT

Keynote speaker, Gamma Sigma Delta annual meeting, Storrs, CT

Keynote speaker, UCONN 50th Class Reunion, Storrs, CT

Keynote speaker, Beijing International Science and Technology Week, Beijing, China

 

2001

Keynote speaker, International Conference on Agricultural Science and Technology, Beijing, China

Featured speaker, Yangling Agricultural Forum, Shanxi, China

Premier lecture, China Dairy Industry, Beijing, China

Governor lecture, Shandong Province, Jinan, China

Governor lecture, Hebei Province, Shijiazhuang, China

Keynote speaker.  Biotechnology and applications in agriculture.  Third World Agricultural Conference.  October 2001, Beijing, China.

Keynote speaker (with cover photo), Applying advanced biotechnology to accelerate China’s dairy industry. Second China High-Tech Forum, November 2001, Yangling, China.

Premier lecture, Ideas to develop China dairy industry (private lecture to Vice Premier Wen Jiabao of China),  November 2001, Beijing, China.

Invited lecture (award ceremony for honorary professor).  Applications of biotechnology in agriculture.  October 2001, Shanghai, China.

Keynote Lecture on animal biotechnology at Connecticut Organ Preservation Society Annual meeting, March 2001. Hartford, CT.

 

2002

Invited lecture, Cloning and genetic engineering of animals. Public Lecture Series, Issues in Biotechnology. April 2002, Connecticut College.

Invited lecture.  The story of cloning.  Public lecture on science and religion. February 2002, Hartford.

Key presentation (Reproduction Leader) followed by panel discussion.  Functional genomic study areas in reproduction.  “Alerton III Conference:  Beyond Livestock Genomics – A Roadmap for Harvesting the Promise.”  (Sponsored and organized by USDA) July 2002

Keynote speaker.  X-chromosomal Inactivation “US-Japan Symposium on Genomic Function for Embryogenesis”, Tokyo, Japan 10/2002

Keynote speaker.  Nuclear Reprogramming after Cloning.  “International Symposium on        Embryo Biotechnology and its Applications”, Seoul, Korea 11/2002

Keynote speaker.  Cloning – State of the Research.  “2002 Symposium on Single Cell Molecular Technology”.  Tokyo, Japan 11/2002

2003

Invited speaker, Activated Egg Symposium, Harvard University

Keynote speaker, Hartford Medical Society annual meeting, Hartford

Plenary lecture.  Animal Cloning and Applications.  “Beijing Nobel Day – Life Science Forum” (included 4 Nobel Laureate speakers)

Invited speaker, First New England Symposium on Regenerative Biology and Medicine, Storrs

Invited speaker, International Symposium on Biotechnology, Hungary

Invited speaker, NRC Symposium on Antiterrorism and K-7 Detection. DC

Invited panelist and initiator, Future Directions and Research Priorities for the USDA Biotechnology Risk Assessment Research Grants Program

 

GRANT SUPPORTS AND FUND-RAISING EXPERIENCE:

 

Grant Supports at UConn (total supports: approximately $6,336,000)

 

-                      Bovine Genetics (USDA)

PI:. X. Yang

            Period (Amount) 1/1/02 ($760,000/year 2002 and $1,000,000/year 2003, renewable)

 

-           Analysis on cloned embryos and placentas in cattle by DNA microarrays (USDA)

PI: X. Yang, X. Tian, H Lewin and M Band

Period (Amount): 10/15/01 - 10/14/04 ($200,000)

 

-                        New Paradigm of Pre-implantation genetic diagnosis (Atlanta Reproduction Associates)

              PI: X Tian and X Yang

              Period (Amount): 06/01/03 - 05/31/04 ($26,000, annual renewable)

 

Commercialization of cattle cloning and related biotechnologies in Connecticut (CII)

        PI: X. Yang, Co-PIs: X Tian, M Taneja

 Period (Amount): 07/01/01-06/30/03 ($300,000)

 

-                      Cryopreservation Of IVP Embryos (Evergen Biotechnologies, Inc.)

            PI: X. Yang

            Period (Amount): 07/01/01-06/30/03 ($100,000)

 

-              Patterns and expression levels of imprinted genes in clones from adult cattle (NIH, 1RO3HD40889-01)

               PI: X Yang, Co-PI: X. Tian

               Period (Amount): 08/01/01-07/31/03 ($143,000)

 

-              Identification Of Expressed Polymorphisms And Imprinted Genes In Cattle (USDA, 01-02402)

               PI: X Yang, Co-PI: X Tian

               Period (Amount): 09/01/01-08/31/03 ($74,996)

 

-              Mammalian oocyte manipulation (Clínica E Centro De Pesquisa Em Reprodução, Brazil)

               PI: X Tian, Co-PI: X Yang

               Period (Amount): 10/1/01- ($12,000/Year, Annually Renewable)

 

-              Reprogramming Of X-Linked Genes By Nuclear Transfer (NIH, 1RO3HD42625-01)

               PI: Tian, Co-PI: X Yang

               Period (Amount): 08/01/02-07/31/04 ($143,000)

 

-                      Cat Cloning and Gene Targeting (Transgenic Pets LLC)

PI:  X. Yang

Period (Amount): 7/1/00 - 9/30/01 ($138,000)

 

            -           Production of transgenic mice (Alexion Therapeuticals, Inc.)

PI: X. Yang

Period (Amount): 01/01/00-12/31/01, ($23,000)

 

-                      US-South Africa Joint Fund (Student Fellowship, USDA)

PI: X. Yang (For Lucky Nadambale)

Period (Amount): 08/15/00- ($28,000/year)

 

            -           Production of knockout mice (RoundTree Biotechnologies, Inc.)

                        PI: X Yang

                        Period (Amount): 7/1/00-indefinite ($46,560)

 

-                      Do Clones from Adult Somatic Cells Suffer Pre-Mature Aging (UCRF)

            PI: X. Yang, X. Tian

Period (Amount): 1/15/00 - 1/14/01 ($19,206)

 

-           Transgenic Rabbit Model for Wound Healing (Hartford Hospital)

PI: G. Perdrizet, L. Hightower, X. Yang

Period (Amount): 1/1/00 - 1/1/01 ($50,000)

 

-           Maximize Viable Embryo Production from Pre- and Peri -Pubertal Calves (USDA)

PI:  X. Yang

Period (Amount): 10/1/96 - 9/30/99 ($180,000)

 

-                      Production of Transgenic Dutch-Belted Rabbits (Genzyme Transgenics Corp.)

PI:  X. Yang

Period (Amount):  11/15/98 - ($428,000)

 

-                      Production of Transgenic NZW Rabbits (Genzyme Transgenics Corp.)

            PI: X. Yang

            Period (Amount): 10/1/98 – ($125,000)

           

-                      Oocyte Competence of Pre-Pubertal Heifers (Genzyme Transgenics Corp.)

PI:  X. Yang

Period (Amount):  5/1/97 – 4/30/99 ($346,080)

 

            -           Production of Cloned Pigs from Embryonic Cell Lines (Biotech Res. Dev. Corp.)

                        PI:  X. Yang, J. Riesen, T. Hoagland

                        Period (Amount):  8/1/99 - 7/31/01 ($461,133)

 

-                      Cloning Pigs – An Ideal Approach to Generate Organs for Transplantation in Humans (CII)

            PI:  X. Yang, J. Riesen, T. Hoagland, T. Chen

                        Period (Amount):  1/15/99 – 1/14/2001 ($294,660)

 

-                      Novel Approach to Produce Transgenic Pigs for Xenotransplantation (CII)

PI:  T. Hoagland, J. Riesen and X. Yang

Period (Amount): 9/1/98 – 8/31/00 ($200,000)

 

-                      Developing ES Cell Lines in Pigs (Hatch)

            PI: X. Yang

            Period (Amount): 9/1/96-8/31/00 (~$280,000)

 

            -           Combined Embryo Biotechnologies – A Viable Business in Connecticut (CII)

PI: X. Yang, Co-PIs: M. Taneja, X. Tian and A. Dinnyes

                        Period (Amount): 7/1/99-6/20/01 ($289,774)

 

-                      China Bridges Fellowships (Rockefeller Foundation)

PI: X. Yang

Period (Amount): 4/1/97 – 3/31/02 ($400,000)

 

-                      China Bridges Fellowships (Lingnan Foundation)

PI: X. Yang

Period (Amount): 4/1/97 – 3/31/02 ($200,000)

 

-                      Exploring a Novel Approach for Producing Transgenic Cattle (UCRF)

PI: X. Yang

Period (Amount): 1/1/97 – 12/31/97 ($16,651)

 

-                      Detection of Transgenic Pre-Implantation Embryos in Mammals (UCRF)

PI:  T. Chen, Co-PI:  X. Yang

                        Period (Amount): 1/1/97 – 12/31/97 ($5,000)

 

Projects Pending:

-                      Comparative gene expression profiling of placental development in farm animals - USDA/NRI

PIs: X Tian and X Yang

Period (Amount): 9/1/03 – 8/30/06 ($760,000)

 

                        National Resources Center on Larger Animal Models for Cardiovascular Diseases (CVD) Research (USDA)

PIs: X Yang and Gary H. Gibbons

Period (Amount): 9/1/04 – 8/30/07 ($9,000,000)

 

 

Previous Supports (total previous supports: approximately $2,750,000)

 

-           Embryo Biotechnologies and Animal Breeding (Eastern A.I. Cooperative, Inc., EAIC)

PI:  X. Yang (has been receiving funding from EAIC since 1991)

Period (Amount): (yearly competitive renewal)

                        8/1/91 - 7/31/96 ($187,500, direct cost)

 

-           Control of Oocyte Maturation, IVF and Embryo Development (NIH). 

PI:  R. H. Foote, X. Yang (one of 4 co-PIs)

Period (Amount): 10/1/91 - 9/30/96 ($1,038,063)

 

-           Combined Embryo Technologies -- From Laboratory to Real World (Cornell Biotechnology Center)

PI: X. Yang

Period (Amount): 7/1/95 - 6/30/97 ($60,000, direct cost)

 

-           Production of Transgenic Rabbits by Microinjection (PPL Therapeutics, Inc., Blacksburg, VA)

PI: X. Yang

                        Period (Amount):  9/15/94 - 9/14/95 ($35,000)

9/15/95 - 9/14/96 ($90,053)

 

-           Production of Viable Transgenic Cattle with Oocytes Collected from Live Cows and Heifers (PPL Therapeutics Inc., Blacksburg, VA)

PI:  X. Yang 

Period (Amount): 9/15/95 -9/14/97 ($192,982)

 

-           Production of Transgenic Rabbits to Produce Human Pharmaceutical Proteins in Milk (GenePharming BV, The Netherlands)

PI: X. Yang

Period (Amount): 3/15/96 - 9/15/96 ($60,500)

 

-           Production of Transgenic Rabbits to Produce Human Pharmaceutical Proteins in Milk (Genzyme Transgenics, Corp.)

PI: X. Yang

Period (Amount):  5/15/96 - 11/15/96 ($60,500)

 

-           Improving Development of IVF Bovine Embryos (Advanced Reproduction Technologies Inc., Spokane, WA)

Collaboration and Consulting Services to Dr. Joanna Ellington

Period (Amount):  10/1/95 - 9/30/96 ($10,300)

 

-           IVF and Embryo Development in Woodchucks (Cornell Veterinary College)

Collaboration and Embryological Services to Dr. Bud Tennant

Period (Amount): 1/1/94 – 5/30/96 ($25,000 direct cost)

 

-           Cloning Cattle with Novel Approaches (Cornell Biotechnology Center)

PI:  X Yang

Period (Amount): 7/1/93 - 6/30/95 ($60,000)

 

-           Polarity, Differentiation and Developmental Potential of Embryonic Cells (USDA)

PI:  X Yang (RH Foote was PI initially, Yang became PI in 1993)

Period (Amount): 10/1/91 - 3/31/96 ($196,239)

 

-           Mechanisms of Endometritis and Infertility in Cattle - An In Vitro Model (Cornell Veterinary College)

PI:  R. Gilbert, Co-PIs: D Schlafer and X Yang

Period (Amount):  1/1/93 - 12/31/93 ($20,288)

 

-           Effect of Inflammatory Reaction on Embryonic Development -- In Vitro Model (Cornell Veterinary College)

PI:  R. Gilbert, Co-PI: X Yang

Period (Amount):  1/1/95 - 12/31/95 ($18,650)

 

-           Toward Developing Emerging Biotechnologies (Cornell CAT Biotechnology)

PI: R. H. Foote, Co-PI: X. Yang

Period (Amount): 7/1/91 - 6/30/93 ($58,000)

 

-           Human IVF and Embryo Culture (Consulting Scientific Director, Baylor Center for Reproductive Health)

PI:  X. Yang (Consulting for Human IVF and Embryological Services)

Period (Amount): 12/1/93 - 12/31/94 ($65,000)

 

-           Functional Enucleation of Bovine Oocytes (US-Hungarian Joint Fund Program)

PI: A. Kovacs, Co-PI: X. Yang

Period (Amount): 4/15/92 - 4/14/95 ($74,000)

 

-           China-Cornell Fellowship Programs (Rockefeller Foundation)

PI: X. Yang, Co-PIs: Y. Shi, D. Wang, X. Zhang

Period (Amount):  12/1/91 - 11/30/97 ($350,000)

 

-           China-Cornell Fellowship Programs (Lingnan Foundation)

PI: X. Yang

Period (Amount):  4/1/95 - 3/31/97 ($100,000)

 

-                      Publication for the Journal of Chinese Agricultural Association of Students and Scholars (Rockefeller Foundation)

PI: X. Yang

Period (Amount):  12/15/91 - 12/14/94 ($23,000)

 

TEACHING RESPONSIBILITY AT UCONN (since 1996):

 

            I have developed the following new courses at UCONN:

 

-          ANSC 360: Frontiers in Animal Biotechnology (graduate level, every other year, 2 credits)

 

The objectives of this course are 1) to provide the Animal Science graduate students with an overview of recent developments in animal biotechnology; 2) to improve graduate students' presentation skills.  After extensive review and discussion of various biotechnologies, each student was asked to give a presentation in the area other than their own research.  I met with all students individually several times during their literature search, preparation of presentation outline and presentation practice.  The course was very well received with a very positive evaluation from all participating students.

 

-          AS 229: Animal Embryology and Biotechnology (undergraduate level, 3 credits)

 

This is the second new course I developed at UConn.  The same well-established format for ANSC 360 is applied to this undergraduate course.  An overview of fundamental mammalian embryology and developmental biology is provided, followed by extensive discussion of the recent fascinating embryological and biotechnology research.  Lab demonstrations and hands on experience are also provided.

 

-          ANSC 397: Animal Science Seminar Series

 

      I have served as the coordinator for the departmental Seminar Series since 1997; My new initiatives for more graduate students' involvement via a students-speaker luncheon have been very well received.  The seminar attendance has increased approximately 50%.

 

-          Mini-courses (workshops): I offered the following mini-courses or workshops at UCONN:

 

a)       OPU/IVF Workshop.  Approximately 20 UCONN staff, students and 3 scientists from other institutions attended this workshop.  Responses from the participants are very positive.

 

b)      Mini-Course on Cloning: The Science and Art of Cloning.  Offered to UCONN undergraduate honors programs.

 

c)      UCONN Mentor Connection.  I have participated in the Mentor program as an instructor for 4 years (summers) and served as the Animal Science coordinator for the program in 1999 and 2000 (3 weeks).

 

d)      4-H Teen Conference (Workshop) and others.  I have offered several workshops for the 4H club, the FFA, and many high school student groups.

 

-          I have served as a guest lecturer for the following courses:

 

INTD 182              Brews and Stew, Spuds and Duds- People, Pestilence and Poverty

MCB 212                 Genetic Engineering

ANSC 120               Introduction to Animal Science

ANSC 219               Reproductive Physiology

PLSC 298/305         Introduction to Biotechnology

 

-          In addition to classroom teaching, I have been supervising about 8-10 student workers and researchers (student research projects) each year at UCONN since 1996.  Currently, I am supervising 10 undergraduate students, 14 graduate students, 5 post-doctoral scientists and 4 research assistants and 2 visiting scientists in my group.

 

TEACHING EXPERIENCE AT CORNELL:

 

I have taught for 4 years as the instructor or co-instructor of the following courses:

 

-     AS431: Embryo Biotechnologies (2 credits).

 

-     BS101: Exploration in Biological Sciences (3 credits)(Sections of Cell Replication and Infertility)

 

-     AS430: Artificial Insemination and Embryo Transfer in Farm and Companion Animals (2 credits)(served as a TA with Dr. Foote for 3 years)

 

In addition, from 1991 to 1996, I have supervised 20 undergraduate students for their research projects and honors thesis projects.  One of the undergraduate students (Lynne Moraghan) I supervised won the 1992 student research competition at the 1992 International Embryo Transfer Society (IETS) meeting.  I have also trained 2 Ph. D. students and 12 post-doctoral and visiting scientists (see list of trainees).

 

SUPERVISING RESPONSIBILITY:

 

Graduate Students Supervised:

 

            Bin Wu                                                  Ph. D.               1992-1995         Cornell University

            Jyh-Cherng Ju                                       Ph. D.               1994-1998         Cornell University

            Pinglei Zhou (Asso. Advisor)                   Ph. D.               1996-2000         Univ. of Connecticut

            B.-Seon Jeong                                        Ph. D.               1996-2003         Univ. of Connecticut

            Jack Xu                                                            Ph. D.               1997-2002         Univ. of Connecticut

            Mark Tripp (Lab. Advisor)                      Ph. D.               1997-2000         Univ. of Connecticut

            Sharmeen Jones                                     M.S.                 1998-2002         Univ. of Connecticut

            Jong Won Lee                                        Ph.D                 1999-2003         Univ. of Connecticut

            Daniela Fischer (transferred)                   M.S.                 1999-2000         Univ. of Connecticut

            Brian Enright                                          Ph.D.                1999-2003         Univ. of Connecticut

            Chikara Kubota (joint training)                 Ph.D.                1999-2003         Univ. of Kogoshima

            Lunar He                                               M.S.                 2002-2003         Univ. of Connecticut

            Joanna Slisz (Res. Advisor)                     M.S.                 1999                 Univ. of Connecticut

            Fei Xue                                                 Ph.D.                2000-                Univ. of Connecticut

            Tshimangadzo Nedambale                       Ph.D.                2000-                Univ. of Connecticut

            Sadie Smith                                           Ph.D.                2001-                Univ. of Connecticut

            Charlie Bormann                                     Ph. D.               2001-                Univ. of Connecticut

            Jeremy Chang                                        Ph.D.                2001-                Univ. of Connecticut

            Le Jiang                                                Ph.D.                2001-                Univ. of Connecticut

            Sanjeev Chaubal                                     Ph.D.                2001-                Univ. of Connecticut

            Li Wang (visiting student)                       Ph.D.                2001-                Univ. of Connecticut

            Lan Yang                                               Ph.D.                2001-                Univ. of Connecticut

            Liying Sung                                           Ph.D.                2003-                Univ. of Connecticut

            Chul Kim                                               Ph.D.                2003-                Univ. of Connecticut

 

Post-doctoral and Visiting Professors Supervised:

            Wancun Chang                                      Ph.D.                5/2002-

            Fuliang Du                                             Ph.D.                8/2000-

            Shouquan Zhang                                    M.S.                 1/2002-4/2003

            Yuqin Zhang                                          B.S.                  2/2002-

            Xianzhi Hou                                           Ph.D.                10/2001-12/2001

            Humphrey Humada                                 Ph.D.                5/2001-7/2001

            John Orris                                             M.D.                 5/2001-11/2002

            David Schmidt                                       M.D.                 11/2002-5/2004

            Ji-long Liu                                             Ph.D.                7/2000-7/2002

            Mikiko Saito                                          Ph.D.                4/2000-12/2000

            Man-qi Deng                                          Ph.D.                8/1999-5/2001

Terry Wu                                              Ph.D.               

Cindy Tian                                             Ph.D.                2/1998-8/2002

Michele Barber                                       Ph.D.                1/1999-5/2001

            Maneesh Taneja                                     DVM/Ph.D.       4/1997-8/2001

            Ji Wu                                                    Ph.D.                1999

            R.S. Manik                                            Ph.D.                6/2000 -12/2000

            Jyh-Cherng Ju                                       Ph.D.                6/2000 - 8/2000

            Andras Dinnyes                                      DVM/Ph.D.       7/1997 –8/1999

Peter Bols                                              DVM/Ph.D.       4/1997 –10/1998

            Howard Levine                                       DVM                1997 – 1998

            Lin Liu                                                  Ph. D.               1996 –1998

            Bin Wang                                              Ph. D.               1995 – 1997

            Gorgio A. Presicce                                 DVM/Ph.D.       1992-1993, 1995 – 1996

            Elena Senatore                                       Ph. D.               1995 – 1996

            Chikara Kubota                                      DVM                1995, 1998, 2002, 2003

            Hiroyuki Suzuki                                      Ph.D.                1995, 1997, 2002-2003

            Neelam Gupta                                        DVM/Ph.D        1999

            S.C. Gupta                                            DVM/Ph.D        1999

 

Other Research Personnel Supervised (Technical Staff/Visiting Scientists):

            Marina Julian                                         1996-

            Teresa Samuels                                      1998-2001

            Shie Jiang                                              1990-

            Li-Ying Sung                                         6/2000-08/03

            Yunping Dai                                           4/1997-7/2000

Anne Van de Velde                                 1997-1998

            Perng-Chin Shen                                    1999

            Z. S. Shi                                                1992-1993

            Abidine Mahhoubi                                   1993

            Laslo Dezso                                           1995

            Fuliang Du                                             1994-1996

            Mel Schultt                                            1995-1996

            Michele Lastro                                       1994-1995

            Suzane Huang                                        1998

            Meijuann Chen                                       1998

 

      Other Staff supervised (China Bridges Fellowship Program)

            Shi Peng                                                2001-2002

            Lisa Li (CBI)                                          1999-2001

            Eric Yang (CBI)                                     2000-2003

            Ying Liu                                                2000-2001

            Jen Dassouki                                         1997-1998

Weimin Zhang (CBI)                               1998-1999

            Hua Hua (CBI)                                       1999

            May Liu                                                2002-

 

COLLABORATIONS:

My laboratory has had collaborations with several groups at Cornell, UConn and elsewhere. Collaborators at UConn include:

-          Animal Science: Drs. Tom Hoagland, John Riesen (Xenotransplantation), Dr. Shiela Andrew (Nutrition and Reproduction), Steve Zinn and Gary Kazmer (Growth and Lactation), Bob Milvae (Endocrinology) and Nissim Yonash (Molecular Genetics), Mike Darre (Behavior);

-          Biological Engineering: Drs. Martin Fox (Ultrasound), Nejat Olgac and Bi Zhang (Biomedical Engineering and Manufacturing);

-          Molecular and Cell Biology: Dr. Thomas Chen (Transgenic Technology), Drs. Larry Hightower and George Perdrizet (UConn Health Center, transgenic rabbit model for wound healing), Mike O’Neill (imprinting).

-          Pathobiology: Richard French (pathology of clones)

 

Collaborations at Cornell University include:

-          The study on molecular regulation and cell cycle control of oocyte maturation and activation (with Dr. Bruce Currie, Animal Science);

-          Cytological analysis of oocyte maturation in cattle (with Dr. John Parks, Animal Science);

-          Oocyte maturation and fertilization in horses (with Drs. Barry Ball and Joanna Ellington, College of Veterinary Medicine) and in woodchucks (with Drs. Patrick Concannon and Bud Tennant, College of Veterinary Medicine);

-          Recently, we have initiated a collaboration project with Drs. Jonathan Hill and Don Schlafer on the histopathology of cloned animals.

 

Other academic collaborations include the following:

-          Ultrasound-guided oocyte retrievals in cattle (with Dr. Charles Looney, Transova Genetics, Sioux Center, IA);

-          Functions of cumulus cells during bovine oocyte maturation and fertilization in vitro (with Dr. Robert Godke, Louisiana State University);

-          Ttransgenic and knockout technology (with Dr. Tom Wagner, Clemenson University);

-          Functional genomics in cloned animals (with Drs. Harris Lewin and Mark Band, University of Illinois);

-          Clone development and abnormalities (with Jean-Paul Renard and Isabelle Hue, INRA, France)

-          Cloned pig telomere reprogramming (with Randy Prather, University of Missouri);

-          Electron microscopy and immunohistochemistry (with Dr. Hiroyuki Suzuki, Hirosaki University, Japan)

-          X chromosome inactivation (with Lygia Perira, University of Sao Paulo, Brazil);

-          Oocyte activation (with Alex Evans and Pat Lonergon, University College Dublin, Ireland);

-          Imprinting in cloned pigs (with Irina Polejaeva, PPL Therapeutics, Inc.)

 

We also have extensive collaboration with animal and transgenic industries, which include:

-          Genex Inc. of Ithaca, NY (Dr. John Thompson);

-          Alexion Pharmaceuticals of New Haven, CT (Drs. Bill Fodor and Ken Bondiolli);

-          PPL Therapeutics of UK (Dr. Alan Colman) and Blacksburg, VA (Dr. Ray Page and Dr. Will Eyestone);

-          Genzyme Transgenics of Framingham, MA (Drs. Carol Ziomek, Yann Echelard, Fritz Reinhart and Li-how Chen, etc.);

-          Pharming BV of the Netherlands (Drs. Frank Pipper and Gerben Moolhuizen);

-          Biotechnology Research and Development Cooperation (Dr. Grant Brewin);

-          TransOva Genetics (Dr. Dave Faber);

-          Em Tran Inc. (Dr. John Hasler);

-          Baylor Center for Reproductive Health - Human IVF (Drs. Sam Marynick and Mike Putman);

-          In Vitro Sciences - A Women Health USA Company (Drs. John Nelson, Vicky Baldwin).

 

PROFESSIONAL MEMBERSHIP:

 

American Association for the Advancement of Science (AAAS)

American Society for Reproductive Medicine (formerly American Fertility Society)

International Embryo Transfer Society (IETS)

Society for the Study of Reproduction (SSR)

The New York Academy of Science

Gamma Sigma Delta (The Honor Society of Agriculture)

Sigma Xi (The Honor Society of Science)

Chinese Association for Science and Technology (CAST)

Society for Chinese Bio-Scientists in America (SCBA)

Association of American Chinese Professors (President, 1997-1999)

 

PUBLIC SERVICES AND LEADERSHIP:

 

Ad hoc reviewer for Nature Genetics, Nature Biotechnology; Proc. Natl. Acad. Sci. (USA); Aging Cell; Biology of Reproduction; Journal of Reproduction and Fertility; Molecular Reproduction and Development; Theriogenology; Journal Experimental Zoology; Reproduction, Nutrition and Development; Trends in Biotechnology; FEBS Letter; USDA Competitive Grants Program and DOC Advanced Technology Program (Experts’ Panel), NSF.

 

Coordinator: (11/03) Nature Special issue in China

Editor-in-Chief (1990-1994), The CAASS Journal

Theme Editor for special issue of Cloning, Engineering in Medicine and Biology, 2003

Theme Editor for special issue of regenerative biology, Reproductive Biology and Endocrinology, 2003

Editorial Board Member, Cell Research (1999-); Advances in Reproduction (2001/2)

Reviewer and Judge for Graduate Student Research Competitions, IETS (1993-1998)

 

Consultant for Genzyme Transgenics Inc., Framingham, MA; Baylor Center for Reproductive Health, Dallas, TX; PPL Therapeutics, Blacksburg, VA; GenePharming BV The Netherlands; Trans Ova Genetics, Sioux, IA; and the Department of Science and Technology, Ministry of Agriculture of China; External Review Council Member; The Chinese Academy of Sciences; Chinese National Institute of Biological Sciences (NISB); National Center on Biotechnology Development; External Advisor, Yangling National Demonstration Zone of Agricultural Hi-Tech Industry, China.

 

Organizer or committee chair for national, international or local scientific symposia:

-          The 1st M.C. Chang Memorial Symposium (Scientific Committee, 1992);

-          Local Chair (1992), the R.H Foote Symposium on Reproductive Physiology;

-          The 1st (1997) and the 2nd (1998) International Congress of Transgenic Animal Technologies;

-          Conference Chairman (1998) of the international conference “Transgenic Production of Human Therapeutics”;

-          Organizer and Instructor (1998) of the Transgenic Animal Facility OPU Workshop;

-           Plenary Session Chair (1997) of the 1st Symposium of the Young Chinese Reproductive Biologists;

-          Symposium Co-Chair and US Agricultural Biotechnology Delegation Coordinator (1999), Sino-US Symposium on Agricultural Biotechnology (Xi’an);

-          Program Committee member and plenary session chair (1999); International Symposium on the 21st Century China (Washington DC);

-          Organizer (2000), Animal Cloning and Biotechnology Workshops for the Connecticut Teen Conference, the UCONN Mentor Connection programs, and the UCONN Honors program.

-          Co-chair (2001), International Conference on Agricultural Science and Technology, Beijing.

-          Co-organized (2002) many international conferences.

-          Conference Chair (2003), First England Symposium on Regenerative Biology and Medicine, Storrs.

 

UCONN Committee: served on several university, college and departmental committees:

-          Graduate Committee (1997-2000, Chair, 2000 );

-          Biotechnology Center Advisory Board (1997- );

-          CANR Agricultural Biotechnology Task Force (1997- );

-          CANR International Affairs Committee (1998- );

-          Animal Science Faculty Search Committee (Animal Molecular Geneticist, 1998);

-          Chancellor Search Advisory Committee (1999-2000);

-          China Coordination Committee (2000- , Steering Committee, 2000-);

-          Director, China Fellowship Programs (1996- );

-          Faculty Advisor, the UCONN Chinese Students and Scholars Association (1998-)

-          Research Support Committee to VP for Research and Graduate Education

-          CRB faculty search committees (5 faculty, coordinator) (2001-2002)

-          Life Science Director Search Committee, CSTC (2001-2002)

-          CSTC Advisory Committee (2000-      )

-          VP for Research and Graduate Committee (2001-2002)

-          UConn Dental School Institutional Infrastructure advisory committee (2003-)

-          UConn Technology Transfer and Commercialization advisory committee (2002-)

-          CANR Dean Reappointment committee (2003)

 

Other Leadership Positions Held:

-          Director, China-Cornell Fellowship Programs (1991 - present), Cornell University;

-          Director, China Bridges International (1996 – present) University of Connecticut;

-          President (1988-1989), the Chinese Agricultural Association of Scientists and Scholars, USA (CAASS); (1990-1994, Editor-in-Chief, the CAASS Journal);

-          President (1997-1999), Association of American Chinese Professors (AACP).

 

PUBLICATION: (more than 80 peer-reviewed journal papers):

 

1.       Yang, X. and Foote, R.H.  Production of identical twin rabbits by micromanipulation of embryos.  Biol. Reprod. 37:1007-1014, 1987.

 

2.       Hansel, W., Alila, H.W., Dowd, J.P. and Yang, X.  Control of steroidogenesis in small and large bovine luteal cells.  Aust. J. Biol. Sci. 40:331-347, 1987.

 

3.       Yang, X. and Foote, R.H.  Production of chimeric rabbit from morulae by a simple procedure.  Gamete Res. 21:345-351, 1988.

 

4.       Chen, Y., Yang, X. and Foote, R.H.  Timed breeding in rabbit with fresh and frozen-thawed semen and evidence of acrosome alteration following freezing and thawing. Anim. Reprod. Sci. 18:35-41, 1989.

 

5.       Chen, Y., Li, J., Simkin, M.E., Yang, X. and R.H. Foote.  Fertility of fresh and frozen rabbit semen inseminated at different times is indicative of male differences in capacitation time.  Biol Reprod. 41:848-854, 1989.

 

6.       Yang, X., Zhang, L., Kovacs, A., Tobback, C. and Foote, R.H.  Potential of hypertonic medium treatment for embryo micromanipulation.  II.  Assessment of nuclear transplantation on blastomere isolation, subzona insertion and electrofusion with intact or functionally enucleated oocytes in rabbits.  Mol. Reprod. Dev. 27:118-129, 1990.

 

7.       Yang, X., Chen, Y., Chen, J. and Foote, R.H.  Potential of hypertonic medium treatment for embryo micromanipulation.  I.  Survival of rabbit embryos in vitro and in vivo following sucrose treatment. Mol. Reprod. Dev. 27:110-117, 1990.

 

8.       Yang, X., Chen, J., Chen, Y. and Foote, R.H.  Improved developmental potential of rabbit oocytes fertilized by sperm microinjection into the perivitelline space enlarged by hypertonic media.  J. Exp. Zool. 255:114-119, 1990.

 

9.       Yang, X. and Foote, R.H.  Survival of bisected morulae transferred to synchronous and asynchronous recipients.  Mol. Reprod. Dev. 26:6-11, 1990.

 

10.   Yang, X.  Featured article:  Embryo cloning by nuclear transfer in cattle and rabbits.  Em. Trans. Newsletter 9 (4):10-22, 1991.

 

11.   Foote, R.H. and Yang, X.  Cloning Bovine Embryos.  Reprod. Dom. Anim. 27:13-21. 1992.

 

12.   Heuwieser, W., Yang, X., Jiang, S. and Foote, R.H.  A comparison between in vitro fertilization and microinjection of immobilized spermatozoa from bulls producing spermatozoa with defects.  Mol. Reprod. Dev. 33:489-491, 1992.

 

13.   Heuwieser, W., Yang, X., Jiang, S. and Foote, R.H.  Fertilization of bovine oocytes after microsurgical injection of spermatozoa.  Theriogenology 38:1-9. 1992.

 

14.   Yang, X. and Anderson, G. B.  Micromanipulation of mammalian embryos: principles, progress and future possibilities.  Theriogenology 38:315-335. 1992.

 

15.   Yang, X., Jiang, S., Kovacs, A. and Foote, R.H.  Nuclear totipotency of cultured rabbit morulae to support full term development following nuclear transfer.  Biol. Reprod. 47:636-643. 1992.

 

16.   Yang, X.  Nuclear transfer in cattle and rabbits: A review.  CAASS J. 2:96-116. 1992.

 

17.   Yang, B.K., Yang, X. and Foote, R.H.  Effect of growth factors on blastocyst development of in vitro matured and in vitro fertilized bovine oocytes.  Theriogenology 40:521-530, 1993.

 

18.   Giles, J.R., Yang, X., Mark, W. and Foote, R.H.  Pluripotency of cultured rabbit inner cell mass cells following injection into blastocysts or morulae.  Mol. Reprod. Dev. 36:130-138, 1993.

 

19.   Ellington, J.E., Ball, B.A. and Yang, X.  Binding of stallion spermatozoa to the zona pellucida after co-culture with oviductal epithelial cells.  J. Reprod. Fert. 98:203-208, 1993.

 

20.   Yang, X., Jiang, S. and Foote, R.H.  Bovine oocyte development following different oocyte maturation and sperm capacitation procedures.  Mol. Reprod. Dev. 34:94-100. 1993.

 

21.   Yang, X., Jiang, S., Farrell, P., Foote, R.H. and McGrath, A.B.  Nuclear transfer in cattle: Effect of nuclear donor cells, cytoplast age, co-culture and embryo transfer. Mol. Reprod. Dev. 35:29-36, 1993.

 

22.   Koyama, H., Suzuki, H., Yang, X., Jiang, S. and Foote, R.H.  Analysis of polarity of bovine and rabbit embryos by scanning electron microscopy (SEM).  Biol. Reprod. 50:163-170, 1994.

 

23.   Zhang J.J., Liu, J., Tchabo, J.G., Yang, X. and DeMattina, M.  Zona-opening of hamster oocytes:  A comparative study using macro and micromanipulation methods.  Human Reprod. 9:137-140, 1994.

 

24.   Suzuki H., Yang, X. and Foote, R.H.  Surface characteristics and size changes of immature, in vitro matured and fertilized bovine oocytes.  Mol. Reprod. Dev. 38:421-430, 1994

 

25.   Yang X., Presicce, G.A., Moraghan, L. and Jiang, S. and Foote, R.H.  Synergistic effect of ethanol and cycloheximide on activation of freshly matured bovine oocytes.  Theriogenology 41:395-403, 1994.

 

26.   Presicce G.A. and Yang, X.  Parthenogenetic development of bovine oocytes matured in vitro for 24 hours and activated by ethanol and cycloheximide.  Mol. Reprod. Dev. 38:380-385, 1994.

 

27.   Presicce, G.A. and Yang, X.  Nuclear dynamics of parthenogenesis of young and aged bovine oocytes following combined ethanol and cycloheximide treatment.  Mol. Reprod. Dev. 37:61-68, 1994.

 

28.   Yang, B.K., Yang, X. and Foote, R.H.  Early development of IVM/IVF bovine embryos cultured with or without somatic cells in a simple serum-free medium and different concentrations of CO2 and O2.  J. Reprod. Dev. 40:197-205, 1994.

 

29.   Du F., Jiang, S. and Yang, X.  Beneficial effect of oocyte activation prior to and during nuclear transfer in cattle using in vitro matured oocytes 24 h of age.  Reprod. Nutr. Dev. 35: 703-712, 1995.

 

30.   Liu Z., Foote, R.H. and Yang, X.  Development of early bovine embryos in co-culture with KSOM, taurine, superoxide dismutase and insulin.  Theriogenology 44:741-750, 1995

 

31.   Zhang, L., Jiang, S., Wozniak, P.J., Yang, X. and Godke, R.A. Cumulus Cell Function During Bovine Oocyte Maturation, Fertilization, and Embryo Development In Vitro.  Mol. Reprod. Dev. 40:338-344.  1995.

 

32.   Yang, B.K., Giles, J.R., Yang, X. and Foote, R.H.  Development of In Vitro Matured/In Vitro Fertilized Bovine Oocytes in a Simple Defined (KSOM) Medium.  J. Reprod. Dev. 41:213-218.  1995.

 

33.   Du, F., Giles, J.R., Foote, R.H., Graves, K.H., Yang, X. and Moreadith, R.W. Nuclear Transfer of Putative Rabbit Embryonic Stem Cells Leads to Normal Blastocyst Development.  J. Reprod. Fert. 104:219-223.  1995.

 

34.   Yang, X.  Cellular and molecular regulation of oocyte maturation, activation and fertilization in cattle.  Arq. Fac. Vet. UFRGS Porto Alegre 25: 84-92, 1996.

 

35.   Wu, B., Ignotz, G., Currie, W. B. and Yang, X.  Temporal distinctions in the synthesis and accumulation of proteins by oocytes and cumulus cells during maturation in vitro of bovine oocytes.  Mol. Repro. Dev. 45:560-565, 1996.

 

36.   Concannon, P., Roberts, P., Ball, B., Schlafer, D. and Yang, X., Baldwin, B. and Tennant, B. Estrus, fertility, early embryo development, and autologous embryo transfer in laboratory woodchucks (Marmota monax).  Lab. of Anim. Sci. 47:63-74, 1997.

 

37.   Presicce G.A., Jiang, S., Simkin, M., Zhang, L., Looney, C., Godke, R.A. and Yang, X.  Age and hormonal dependence of acquisition of oocyte competence in pubertal calves.  Biol. Reprod. 56:386-392, 1997.

 

38.   Wu B., Ignotz, G., Currie, W.B. and Yang, X.  Analysis of c-mos proto-oncoprotein and its association with tubulins in bovine oocytes during maturation in vitro.  Biol. Reprod. 56:260-267, 1997.

 

39.   Wu B., Ignotz, G., Currie, W.B. and Yang, X.  Dynamics of maturation promoting factor (MPF) and its constituent proteins during in vitro maturation of bovine oocytes.  Biol. Reprod. 56:253-259, 1997.

 

40.   Suzuki, H., Fujiwara, T. and Yang, X.  Surface ultrastructural characteristics of the hamster oocyte and its investments during in vivo maturation.  J. Mamm. Ova. Res. 14:191-198, 1997.

 

41.   Suzuki, H., Presicce, G.A. and Yang, X.  Differential surface ultrastructural characteristics and volumetric dynamics of bovine oocytes during maturation in vivo versus in vitro. J. Mamm. Ova Res. 15:49-62, 1998.

 

42.   Liu, L., Ju, J-C. and Yang, X.  Parthenogenetic development and protein patterns of newly matured bovine oocytes after chemical activation.  Mol. Reprod. Dev. 49:298-307, 1998.

 

43.   Tian, X.C. and Yang, X.  Life on the bio-pharm: therapeutic proteins form transgenic organisms.  Mol. Med. Today. 4:424-425, 1998.

 

44.   Yang, X., Kubota, C., Suzuki, H., Taneja, M., Bols, P.E.J. and Presicce, G.A.  Control of oocyte maturation in cows - biological factors.  Theriogenology 49:415-508, 1998.

 

45.   Kubota,C., Yang, X., Dinnyes, A., Todoroki, J., Yamakuchi, H., Mizoshita, K., Inohae, S., Tabara, N.  In vitro and in vivo survival of frozen-thawed bovine oocytes after IVF, nuclear transfer, and parthenogenetic activation.  Mol. Repro. Dev. 51:281-286, 1998.

 

46.   Liu, L., Ju, J.C. and Yang, X.  Differential inactivation of maturation-promoting factor and mitogen-activated protein kinase following parthenogenetic activation of bovine oocytes.  Biol. Reprod. 59:537-545, 1998.

 

47.   Taneja M. and Yang, X.  Feature Article: Promises and problems of in vitro production of embryos by TVOR-IVF scheme in cows and heifer. Em. Trans. Newsletter16:10-12, 1998.

 

48.   Suzuki H., Ju, J.-C., Parks, J.E. and Yang, X.  Surface ultrastructural characteristics of bovine oocytes following heat shock.  J. Reprod. Dev.  44:345-351, 1998.

 

49.   Liu, L. and Yang, X.  Interplay of maturation promoting factor and mitogen-activated protein kinase during metaphase to interphase transition of activated bovine oocytes.  Biol. Reprod. 61:1-7, 1999.

 

50.   Ju, J.-C., Parks, J. E. and Yang, X.  Thermotelerance of IVM-derived bovine oocytes and embryos after short-term heat shock, Mol. Reprod. Dev. 53:336-340, 1999.

 

51.   Van de Velde, A., Liu, L., Bols, P.E.J., Ysebaert, M-T. and Yang, X.  Cell Allocation and Chromosomal Complement of Parthenogenetic and IVF Bovine Embryos.  Mol. Reprod. Dev. 54:57-62.  1999.

 

52.   Dinnyes, A., Lonergan, P., Fair, T., Boland, M.P. and Yang, X.  Timing of the First Cleavage Post-Insemination Affects Cryosurvival of In Vitro-Produced Bovine Blastocysts.  Mol. Reprod. 53:318-324.  1999.

 

53.   Suzuki, H., Azuma, T., Koyama, H. and Yang, X.  Development of Cellular Polarity of Hamster Embryols during Compaction.  Biol. Reprod. 61:521-526.  1999.

 

54.   Suzuki, H., Liu, L. and Yang, X.  Age-Dependent Development and Surface Ultrastructural Changes Following Electrical Activation of Bovine Oocytes.  Reprod. Fertil. Dev. 11:159-165.  1999.

 

55.   Tripp, M.W., Ju, J-C., Hoagland, T.A., Riesen, J.W., Yang, X. and Zinn, S.A.  Influence of Somatotropin and Nutrition on Bovine Oocyte Retrieval and In Vitro Development.  Theriogenology 53:1581-1590.  2000.

 

56.   Dinnyes, A., Dai, Y., Jiang, S. and Yang, X.  High Developmental Rates of Vitrified Bovine Oocytes Following Parthenogenetic Activation, In Vitro Fertilization and Somatic Cell Nuclear Transfer.  Biol. Reprod. 63:513-518.  2000.

 

57.   Yang, X., Tian, X-C., Dai, Y. and Wang, B.  Transgenic Farm Animals: Applications in Agriculture and Biomedicine.  Elesevier 5:269-292.  2000.

 

58.   Suzuki H., Jeong, B.S. and Yang, X.  Dynamic changes of cumulus-oocyte cell communication during in vitro maturation of porcine oocytes.  Biol. Reprod. 63:723-729,  2000.

 

59.   Lonergan, P., Dinnyes, A., Fair, T., Yang, X., Boland, M.P.  Bovine oocyte and embryo development following meiotic inhibition with butyrolactone-1.  Mol. Reprod. Dev. 57:204-209, 2000.

 

60.   Enright B.P., Lonergan, P., Dinnyes, A., Fair, T., Ward, F.A., Yang, X. and Boland, M.P.  Culture of in vitro produced bovine zygotes in vitro vs in vivo: Implications for early embryo development and quality.  Theriogenology 54:659-673, 2000.

 

61.   Suzuki H., Ju, J.-C. and Yang, X.  Surface ultrastructural alterations of bovine oocytes after parthenogenetic activation.  Cloning 2:69-78, 2000.

 

62.   Xu, J. and Yang, X.  Telomerase activity in bovine embryos during early development. Biol. Reprod. 63:1124-1128, 2000.

 

63.   Tian, X-C., Xu, J. and Yang, X.  Normal telomere lengths found in cloned cattle.  Nature Genetics 26:272-273, 2000. (with world-wide press release)

 

64.   Yang X and Tian, X-C. Cloning adult animals - What is the genetic age of the clones?  Cloning 2: 123-128, 2000.

 

65.   Kubota, C., Yamakuchi, H., Todoroki, J., Mizoshita, K., Tabara, N., Barber, M. and Yang, X.  Six Cloned Calves Produced from Adult Fibroblast Cells After Long-Term Culture.  PNAS 97:990-995. 2000.  (Commentary – Capecchi, M.R.  PNAS 97:956-957.  2000.) (with world-wide press release)

 

66.   Taneja, M., Bols, P.E.J., Van de Velde, A., Ju, J-C., Schreiber, D., Tripp, M.W., Levine, H., Echelard, Y, Riesen and Yang, X.  Developmental Competence of Juvenile Calf Oocytes In Vitro and In Vivo: Influence of Donor Animal Variation and Repeated Gonadotropin Stimulation.  Biol. Reprod. 62:206-213. 2000.

 

67.   Xu, J. and Yang, X.  Telomerase activity in early bovine embryos derived from parthenogenetic activation and nuclear transfer. Biol. Reprod. 64:770-774, 2001.

 

68.   Dinnyes, A., Liu, L., Dai, Y., Barber, M., Xu, J., Zhou, P. and Yang, X.  Development of cloned embryos from adult rabbit fibroblast cells. Biol. Reprod. 64:257-263, 2001.

 

69.   Deng, M.Q. and Yang, X. Full Term Development of Rabbit Oocytes Fertilized by intracytoplasmic Sperm Injection. Mol. Reprod. Dev. 58:1-6, 2001.

 

70.   Jeong, B.S. and Yang, X. Cysteine, glutathione, and percoll treatments improve porcine oocyte maturation and fertilization in vitro. Mol Repro Dev 59:330-335, 2001.

 

71.   Yang, X. and Xu, J.  Developmental changes of telomerase in oocytes and embryos. Advances in Reproduction 6:63-68, 2001.

 

72.   Tian, X-C., Kubota, C. and Yang, X. Cloning of aged animals - a medical model for tissue and organ regeneration. Trends in Cardiovascular Medicine 11:313-317  2001.

 

73.   Xue, F., Tian, X-C, Kubota, C, Du, F., Taneja, M., Dinnyes, A., Dai, Y., Lewin, H., Pereira, L-V. and Yang, X. Aberrant patterns of X-Chromosome inactivation in bovine clones. Nature Genetics 31:216-220, 2002.

 

74.   Du, F., Sung, L-Y., Tian, X-C. and Yang, X. Differential Cytoplast Requirement for Embryonic and Somatic Cell Nuclear Transfer in Cattle. Mol Reprod Dev 63:183-191, 2002.

 

75.   Liu, J-L., Sung, L-Y., Tian, X-C. and Yang, X. Hypertonicity-Induced Projections Reflect Cell Polarity in Mouse MII Oocytes: Involvement of Microtubules, Microfilaments, and Chromosomes.  Biol Reprod 67:1853-1863 2002.

 

76.   Govoni, K.E., Tian, X-C., Kazmer, G.W., Taneja, M., Enright, B., Rivard, A.L., Yang, X. and Zinn, S-A. Age-related changes of the somatotropic axis in cloned Holstein calves.   Biol Reprod 66:1293-1298 2002.

 

77.    Liu, L., Deng, M., Tian, X-C. and Yang, X. Chapter 17. Principles and Practice of oocyte activation in mammals. In: Introduction to Mammalian Reproduction. Daulat R.P. Tulsiani, Kluwer Academic Publishers, MA, USA, pp 319-345, 2002.

 

78.    Tian, X-C.,Lonergan, P., Jeong, B.S., Evans, A.E.O. and Yang, X.  Association of MPF, MAPK and nuclear progression dynamics during activation of young and aged bovine oocytes.  Mol Reprod Dev 62:132-138 2002.

 

79.    Dinnyes, A., Tian, X-C. and Yang, X. Cloning of Rabbits (Chapter 17).  In: Principles of Cloning. Eds. JB Cibelli, RP Lanza, KHS Campbell and MD West.  Academic Press, San Diego, CA. pp. 344-366, 2002.

 

80.   Liu, J-L., Sung, L-Y., Barber, M. and Yang, X.  Hypertonic Medium Treatment for Localization of Nuclear Material in Bovine Metaphase II Oocytes.  Biol. Reprod. 66:1342-1349.  2002.

 

81.   Enright, B., Taneja, M., Schreiber, D., Riesen, J., Tian, X-C., Fortune, J.E. and Yang, X.  Reproductive Characteristics of Cloned Heifers Derived from Adult Somatic Cells.  Biol. Reprod. 66:291-296.  2002.

 

82.  AF Savage, J Maull, XC Tian, M Taneja, L Katz, M Darre and X Yang. Behavioral Observations of Adolescent Holstein Heifers Cloned from Adult Somatic Cells. Theriogenology 60:1097-1110. 2003.

 

83.      BP Enright, BS Jeong, X Yang and XC Tian. Epigenetic Characteristics of in vitro Cultured Donor Cells for Nuclear Transfer: Levels of Histone Acetylation. Biol Reprod 69:1525-1530.

 

84.   Lee J, Wu S-C, Tian X, Barber M, Hoagland T, Riesen J and Yang X. Production of Cloned Pigs by Whole Cell Intracytoplasmic Microinjection. Biol Reprod 69:995-1001, 2003.

 

85.   Jang-Won Lee, X Cindy Tian and Xiangzhong Yang. Failure of male pronucleus formation is the major cause for the lack of fertilization and embryo development in pig ICSI oocytes. Biol Reprod 68:1341-1347. 2003.

 

86.  BP Enright, C Kubota, X Yang and XC Tian. Epigenetic Characteristics of Donor Cells treated by Trichostatin A or 5-Aza-2’-deoxycytidine and development of embryos cloned from treated donor cells. Biol Reprod 69:896-901, 2003.

 

87.      Lee J, Tian X and Yang X. Optimization of Parthenogenetic Activation Protocol in Porcine. Mol Reprod Dev (in press). 2003

 

88.      Suzuki H, Saito Y, Kagawa N, and Yang X.  In vitro fertilization and polyspermy in the pig: Factors affecting fertilization rates and cytoskeletal reorganization of the oocyte. 61:327-334, 2003

 

89.      Liu JL, Sung LY, Du FL, Julian M, Jiang S, Barber M, Xu J, Tian X and Yang X. Differential Development of Rabbit Embryos Derived from Parthenogenesis and Nuclear Transfer. Mol Reprod Dev (in press), 2003

 

90.      Nedambale TL, Dinnyés A, Groen W, Dobrinsky JR, Tian X and Yang X .  Comparison on in vitro fertilized bovine embryos cultured in KSOM or SOF and cryopreserved by slow freezing or vitrification.  Theriogenology (accepted), 2003

 

91.      Xu J and Yang X.  Will Cloned Animals Suffer Premature Aging - The Story at the End of Clones’ Chromosomes.  Reprod Biol Endo (in press), 2003

 

92.      Tian C, Kubota C, Enright B and Yang X.  Cloning animals by somatic cell nuclear transfer – biological factors.  Reprod Biol Endo (in press), 2003

 

93.      X Yang. Therapeutic Cloning and Embryo-based Research on Biotechnology and Medicine: Opportunities and Challenges for China. Nature (in press) 2003.

 

Miscellaneous publications

  1. Zhou, P., G.W. Kazmer, and X. Yang. 2000. Bos taurus growth hormone releasing hormone (GHRH) mRNA. Genbank submission Accession number NM178325.

 

  1. Zhou, P., G.W. Kazmer, and X. Yang. 2000. Bos taurus growth hormone releasing hormone gene complete cds. Genbank submission Accession number AF242855.

 

  1. Yang, X. and X.C. Tian. 2001. Don't ban therapeutic cloning.  Commentary in Hartford Currant, Dec 2, 2001.

 

  1. Tian, X.C., P. Zhou, N. Abraham, M. O’Neill and X. Yang.  2002. Sequence of 3’-UTR of bovine IGFII receptor cDNA.  Genbank submission Accession number AF416605.

 

Not included in the above list are over 100 abstracts, technical reports or conference proceedings papers.

 

PATENTS AND INVENTION DISCLOSURE

 

1.       Cloning mammals using long-term cultured cells.

US#:  09-755-204

PCT#:  PCT/US/01/00394

 

2.       High Development of vitrified bovine embryos.

US#:  09-755-205

PCT#:  PCT/US01/00395

 

3.   Somatic cell cloning by whole-cell intracytoplasmic injection (disclosure). 

 

RECENT INVITED SYMPOSIUM LECTURES AND SEMINARS (about 20/year)

 

1.       Invited lecture, Cloning and genetic engineering of animals. Public Lecture Series, Issues in Biotechnology.  April 2002, Connecticut College.

 

2.       Invited lecture.  The story of cloning.  Public lecture on science and religion. February 2002, Hartford.

 

3.       Guest lecture.  Gene expression and abnormality in clones.  2002.  University of Illinois, Urbana.

 

4.       Guest lecture.  Gene expression and abnormality in clones.  2002.  USDA Beltville Labs.

 

5.       Invited Lecture.  Bovine cloning- state of the technology and the next 50 years. Dec. 2001. Texas A and M University.

 

6.       Keynote speaker.  Biotechnology and applications in agriculture.  Third World Agricultural Conference.  October 2001, Beijing, China.

 

7.       Keynote speaker (with cover photo), Applying advanced biotechnology to accelerate China’s dairy industry. Second China High-Tech Forum, November 2001, Yangling, China.

 

8.       Premier lecture, Ideas to develop China dairy industry (private lecture to Vice Premier Wen Jiabao of China), November 2001, Beijing, China.

 

9.       Governor lecture, Shandong Province, November 2001, Jinan, China.

 

10.   Invited lecture, Ideas to develop China dairy industry, November 2001, Shandong Academy of Agricultural Sciences.  Jinan, China.

 

11.   Governor lecture, Hebei Province, November, 2001, Shijiazhuang, China

 

12.   Invited lecture (award ceremony for honorary professor).  Applications of biotechnology in agriculture.  October 2001, Shanghai, China.

 

13.   April 2001.  Presentation to Connecticut Innovations Inc., Branford, CT.

 

14.   March 2001. Keynote Lecture on animal biotechnology at Connecticut Organ Preservation Society Annual meeting, Hartford, CT.

 

15.   February 2001.  Invited Lecture on animal biotechnology in Louisiana Consortium in Biotechnology, Baton Rouge, LA.

 

16.   January 2001. Invited Presentation at W-171 Annual meeting on embryo development.

 

17.   Keynote Lectures in numerous Connecticut middle and high schools in 2000 and 2001.

 

18.   December 2000.  Invited Lecture on animal biotechnology in Louisiana Consortium in Biotechnology, New Orleans, LA.

 

19.   December 2000. Invited Lecture.  Shenzhen High Tech Industry Groups on cloning and transgenesis.

 

20.   December 2000. Invited Lecture on biotechnology to Beijing Municipal Government.

 

21.   December 2000.  Sun Yet-sun Lecture.  Animal Cloning and Transgenesis in the 21st Century.  Guangzhou, China.

 

22.   November 2000. Invited Lecture on animal biotechnology in Audubon Society in New Orleans, LA.

 

23.   September 2000. Symposium Co-Chairman and Keynote speaker on animal biotechnology. Joint US-China Agricutural Biotechnology Symposium, Nanjiang, China.

 

24.   September 2000. President Lecture. Current Status of animal biotechnology and implications in biomedicine, Nanjing Agricultureal Univeristy, Nanjiang, China.

 

25.   September 2000. Invited Lecture in Shanghai Academy of Agricultural Sciences. Shanghai, China.

 

26.   September 2000.  Invited seminar.  "Somatic Cell Cloning - Applications in Human Tissue and Organ Generation Therapy".  Women's Health USA.  Avon, CT.

 

27.   August 2000.  Keynote speaker.  "Animal Cloning and Related Biotechnologies in the 21st Century - Opportunities and Challenges".  Symposium on 21st Century China and Globalization.  Beijing, China.

 

28.    August 2000. Keynote speaker.  "Animal Cloning and Related Biotechnologies in the 21st Century - Opportunities and Challenges".  Dalian Symposium on High Technologies.  Dalian, China.

 

29.   August 2000.  Seminar.  "Animal Cloning - Progress and Challenges".  Rutgers University, New Brunswick, NJ.

 

30.   July 2000.  Organizer and instructor.  Workshop on Animal Cloning and Related Biotechnology for CT High School Teachers. Storrs, CT.

 

31.   July 2000.  Keynote speaker.  UCONN Cooperative Extension System 2000 Annual Picnic.  Warren Woods, CT.

 

32.   July 2000.  Invited report on Animal Biotech Research to Chancellor John Pedersen.  Storrs, CT.

 

33.   July 2000. Seminar.  Cloning Adult Animals - Progress and Significance".  Advanced Cell Technology Inc.  Worcester, MA.

 

34.   July 2000. Seminar.  Cloning Adult Animals - Progress and Significance". Geron Inc. Manlo Park, CA.

 

35.   July 2000.  Organizer and instructor.  Workshop on Cloning.  Connecticut Teen Connection Conference.  Storrs, CT.

 

36.   June 2000.  Invited speaker. "What Is the Age of Clones?"  Symposium on Genetically Engineering and Cloning Animals.  Park City, UT.

 

37.   June 2000.  Keynote speaker. "Animal Cloning - Applications to Agriculture and Implications to Biomedicine".  UCONN Class of 1950 Reunion, Storrs, CT.

 

38.   May 2000.  Keynote speaker.  "Animal Cloning - the Battle Field of Biomedicine in the 21st Century". Conference on Biomedicine and Pharmaceuticals in the 21st Century.  Beijing, China.

 

39.   May 2000.  Keynote speaker. "Animal Cloning - State of the Technology and the next 50 Years". International High-Tech Industries Week, 2000.  Beijing, China.

 

40.   May 2000.  Seminar. "Animal Cloning - State of the Technology and the next 50 Years".  Chinese Academy of Sciences.  Beijing, China.

 

41.   May 2000.  Seminar.  "Animal Cloning - Applications to Agriculture and Implications to Biomedicine".  University of Connecticut Medical Center, Farmington, CT.

 

42.   May 2000.  Invited speaker.  "Animal Cloning and Medical Implications".  Symposium on Bioengineering of Farm Animals: Legal and Ethical Issues. New York Bar Association Annual Meeting, New York, NY.

 

43.   April 2000.  Keynote speaker.  "Animal Cloning and Transgenesis for Sustainable Development".  International Symposium on Managing Bio-Resources and Bio-Diversity: Agriculture of the New Century.  Taipei, Taiwan.

 

44.   April 2000.  Seminar.  "Animal Cloning - State of the Technology and the next 50 Years". National Chung Hsing University, Taichung, Taiwan.

 

45.   April 2000.  Seminar.  "Animal Cloning - State of the Technology and the next 50 Years". Taiwan Livestock Research Institute.  Hsin-Hua, Taiwan.

 

46.   April 2000.  Seminar.  "Animal Cloning - State of the Technology and the next 50 Years". Pig Research Institute Taiwan.  Maoli, Taiwan.

 

47.   April 2000.  Seminar.  "Animal Cloning - State of the Technology and the next 50 Years". National Taiwan University. Taipei, Taiwan.

 

48.   April 2000.  Keynote address.  Future Perspective of Biotechnology Research.  Gamma Sigma Delta Annual Meeting, Storrs, CT.

 

49.   April 2000.  Seminar.  Animal Cloning - State of the Technology and the Next 50 Years.  Louisiana State University, Baton Rouge, LA.

 

50.   March 2000.  Invited speaker.  Cloning Cattle: Biological Factors.  Banbury Conference on Mammalian Cloning: Biology and Practice.  Cold Spring Harbor, NY.

 

51.   March 2000.  Seminar.  Animal Cloning - State of the Technology and the Next 50 Years.  MBL, Woods Hole, MA.

 

52.   December 1999.  Keynote lecture.  Genetics Minicourse on animal transgenesis and cloning (CT high school biotechnology class).

 

53.   November 1999.  Keynote lecture.  The Story of Amy.  FFA Annual Meeting.  Woodsberry, CT.

 

54.   October 1999.  Keynote presentation to China Agriculture Delegation headed by Governor of Ningxia Province, China.

 

55.   October 1999.  Guest lecture.  East Lyme high school biotechnology class.

 

56.   October 1999.  Keynote lecture.  UCONN Honors Program minicourse on biotechnology.

 

57.   October 1999.  Invited speaker.  CT Agriculture Information Council meeting.

 

58.   September 1999.  Seminar.  Animal cloning - State of the technology and applications.  Xinjiang University and Xinjiang Academy of Animal Sciences.

 

59.   September 1999.  Keynote speaker.  Conference on 21st Century Life Sciences.  Kunming, Yunnan, China.

 

60.   September 1999.  Invited speaker.  Suggestions on strategically development in agriculture in China.  Meeting with the Chinese deputy premier. Beijing, China.

 

61.   September 1999.  Invited seminar.  Cloning - from Dolly to Amy and the Next 50 Years.  Clontech, Palo Alto, CA.

 

62.   September 1999.  Invited strategic planning seminar.  Cloning for Animal Transgenesis - State of the Technology and the Next 50 Years.  Rutgers University, New Brunswick, NJ.

 

63.   September 1999.  Keynote speaker and plenary session chair.  Cloning - State of the Technology and the Next 50 Years.  International Symposium on the 21st Century China.  Washington DC.

 

64.   August 1999.  Invited speaker.  Cloning Cattle - A Progress Report.  Transgenic Animal Research Conference.  Lake Tahoe, CA.

 

65.   August 1999.  Featured speaker.  Cloning - State of the Technology and the Next 50 Years.  7th Annual Conference of Sino-American Pharmaceutical Association.  New Brunswick, NJ.

 

66.   August 1999.  Invited featured presentation to International Students School Learning Camp (50 High School Students from China).  Production of Amy and its Significance.  Storrs, CT.

 

67.   July 1999.  Invited speaker.  Animal Biotechnology - From IVF to Animal Transgenesis to Cloning.  Symposium on Science, Technology and Sustainable Development of the 21st Century China.  Beijing, China.

 

68.   July 1999.  Symposium co-organizer, and keynote speaker.  Animal Biotechnology – from IVF to Transgenesis to Cloning.  Sino-US Agricultural Biotechnology Symposium, Xi'an, China.

 

69.   July 1999.  Invited presentation to Animal Dairy Advisory Council.  The Successful Production of Amy the Clone. UConn, Storrs.

 

70.   June 1999.  Invited presentation.  The Rabbit for Medical Research. UConn Medical School and Hartford Hospital.  Hartford.

 

71.   May 1999.  Invited seminar.  The Science and Art of Cloning – Past, Present and Future.  Department of Animal Production, University College Dublin, Ireland.

 

72.   May 1999.  Invited seminar. The Science and Art of Cloning – Past, Present and Future. Department of Science and Technology, Bryant College, RI.

 

73.   May 1999.  Invited lecture.  The Impact of Biotechnology on Environment.   Department of Science and Technology, Bryant College, RI.

 

74.   May 1999.  Invited lecture.  The State of Animal Biotechnology Research.  Mont Clair State University.  NJ.

 

75.   April 1999.  Invited seminar.  Animal Cloning – Past, Present and Future.  Department of Physiology and Neurobiology, University of Connecticut, Storrs, CT.

 

76.   February 1999.  Invited seminar.  Transgenic Farm Animals – Problems, Progresses and promises. Department of Pathobiology, University of Illinois, Urbana-Champaign, IL.

 

77.   January 1999.  Invited panelist. “Control of the Ovary”, The 15th Annual Meeting of the International Embryo Transfer Society.  Quebec, Canada.

 

78.   December 1998.  Invited keynote speaker.  Advanced Reproductive Technology and its Applications in Transgenics and Agriculture.  Italian National Agricultural Biotechnology Symposium.  Rome, Italy.

 

79.   December 1998.  Invited seminar.  Regulation of Oocyte Embryonic Competence in Cattle.  University of Milan College of veterinary medicine, Milan, Italy.

 

80.   December 1998. Invited seminar. Regulation of Embryogenic Competence in Prepubertal Heifers and Cows.  Department of Animal and Veterinary Sciences, University of Massachusetts, Amherst, MA.

 

81.   November 1998.  Invited speaker. Reproductive Biotechnology and Its Applications to Animal Breeding.  Connecticut Annual Dairy Meeting. Brooklyn, CT.

 

82.   November 1998. Invited seminar.  Progress in Embryo Manipulations – Medical Applications. Brown University Medical Center.  Providence, RI.

 

83.   November 1998.  Invited seminar. Reproduction of Prepubertal Heifers and Cloning -.A Progress Report.  Charlton, MA.

 

84.   September 1998.  Invited Expert Panelist.  Department of Commerce Advanced Technology Program Electronic Workshop – Genetic Manipulation in Animals: Advanced Transgenesis and Cloning.  Washington, D.C.

 

85.   September 1998.  Invited speaker.  Animal Biotechnology. US-China Agricultural Workshop. Cornell University, Ithaca, NY.

 

86.   January 1998. Invited plenary lecture.  1998 Annual Meeting of the International Embryo Transfer Society, Boston, MA.

 

87.   June 1998. Invited plenary lecture.  Transgenic Production of Human Therapeutics. Waltham, MA.

 

88.   May 1998. Invited presentation (organizer) to the President delegation of Top Chinese Agricultural Universities, Storrs, CT.

 

89.   April 1998. Invited presentation at Chinese Northwestern Agricultural University.

 

90.   April 1998. Invited presentation at Shanghai Institute of Human Medical Genetics.

 

91.   June 1998.  Director and instructor, Workshop on Transvaginal Oocyte pickups.  Storrs, CT.

 

92.   August 1998. Invited presentation to Connecticut Department of Agricultural Advisory Board. Hartford, CT.

 

93.   August 1997. Invited keynote speaker, 1997 Annual National Meeting of the Brazil Embryo Transfer Society.  Fos do Ignasu, Brazil.

 

94.   October 1997. Invited plenary lecture.  First International Symposium of Young Chinese Reproductive Biologists.  Beijing (absent due to illness).

 

95.   June 1998. Invited lecture. Third Connecticut Critical Technology Conference.  Hartford, CT.

 

96.   October 1997. Invited presentation (organizer) to the Governor delegation from Shaanxi Province of China, Storrs, CT.

 

97.   January 1997. Invited seminar: Puberty and Oocyte Competence for Embryogenesis in the Female - a Cattle Model.  Department of Pathobiology, UCONN.

 

98.   February 1997. Invited presentation: Animal Biotechnology. Presentation at the Connecticut Farm Bureau Board meeting held at the College of Agriculture and Natural Resources, UCONN.

 

99.   February 1997.  Invited presentation: Reproductive and Transgenic Research at UCONN.  Presentation at the UCONN Board of Trustees meeting, Stamford, CT.

 

100.   March 1997. Invited lecture: Undergraduate Honors Seminar (Bio 196).  Transgenic Animals - Theory and Techniques, UCONN.

 

101.   April 1997. Invited lecture: Annual Academic Day.  Animal Biotechnology Research in Animal Science. UCONN.

 

102.   March 1997. Invited presentation: To delegation from Genzyme.  Keys for Developing a Successful Transgenic Animal Program.  UCONN Biotechnology Center.

 

103.   April 1997. Invited speaker: A Biotechnology Symposium: From Benchtop to Market-place, Washington, DC.

 

104.   November 1996.  Special keynote lecture: Transgenic Farm Animals - Applications in Agriculture and Implications in Biomedicine.  3rd National Congress of Japanese Embryo Transfer Society.  Hirosaki, Japan.

 

105.   November 1996. Invited seminars: Transgenic Farm Animals - Problems and Promises. Kinki University, Japan.

 

106.   November 1996. Invited seminars: Transgenic Farm Animals - Problems and Promises. Osaka University, Japan.

 

107.   November 1996. Invited seminars: Transgenic Farm Animals - Problems and Promises. Kagoshima University, Japan.

 

108.   November 1996. Invited seminars: Transgenic Farm Animals - Problems and Promises. Rakuen University, Japan.

 

109.   June 1996.  Invited speaker.  Transgenic and Related Biotechnology in Animal Science.  Joint Symposium of UCONN at Storrs and UCONN Medical Center.  Storrs, CT.

 

110.   August 1996.  Invited speaker:  Transgenic Farm Animals:  State of the Technology. Connecticut Critical Technology Symposium, Watertown, CT.

 

111.   March 1996.  Invited seminar (offered a faculty position). Genetic Manipulations of Embryos: Applications in Animals and Implications in Humans.  Animal Science/Biotech Center, Rutgers University.  New Brunswick, NJ.

 

112.   March 1996.  Invited seminar (offered a faculty position).  Genetic Manipulations of Embryos: Applications in Animals and Implications in Humans.  Animal Science/Biotech Center, University of Connecticut.  Storrs, CT.

 

113.   April 1997. Invited seminar: Shanghai Society of Genetics and Shanghai Institute of Medical Genetics: Genetic Manipulation of Mammalian Embryos: the Application in Agriculture and Biomedicine. Shanghai, China.

 

114.   January 1997. Invited seminar:  Animal Biotechnologies - State of the Technology and its Application in Agriculture and Medicine. Pfizer Central Research, Groton, CT.

 

115.   May 1997. Invited presentation: Genzyme Transgenic Corp.: Progress Reports and New Proposals on Transgenic Cattle and Rabbits.  Framingham, MA.

 

116.   In addition, my lab receives each year hundreds of visitors, including scientists, students, teachers, farmers and tour groups around the world.