Sure You Can Stomach This?
What's Next?
The next obvious group of tissues to tackle is the
intestines and all other organs which are attached for the ride.
1. Sever the intestines
from the body as far down as possible on the colon.
2. If you want to preserve the rectum and colon,
it may be beneficial if you simply cut the skin around the anus.
3. Free the intestines from any fatty anchors
until you reach the stomach.
4. The caudate lobes of the liver are going
to beon the lateral sides of the stomach. Careful cutting of the
connective tissue is required to leave the liver intact.
5. Cut the esophagus directly above the stomach
in the abdominal cavity, leaving 0.25-0.5 cm of esophagus left
connected to the stomach. At this point the whole kit and kaboodle
can be removed from the abdominal cavity.
Intestines
Starting at the rectum/colon, unzip the coiled
intestines by pulling gently, using your scissors for help whenever
you feel it is necessary . As you do so, check the surface of
the intestines for enlarged Peyer's patches.There are many different
parts of the intestine. Unfortunatly the large majority of them
cannot be differentiated from each other grossly.
Pancreas/Spleen
1.At one point,
the ease at which the intestines can be pulled apart will change
as you hit the pancreas. The pancreas is tan in color and can
be easily identifiable from the surrounding fat. First cut the
stomach away at the duodenal junction and remove the spleen, placing
the spleen in preservative.
2. Cut a section of the small intestine
the pancreas is attached to, using the intestine as a handle.
3. Spread the pancreas out flat on
a piece of paper towel. Within the tissue, lies the pancreatic
lymph nodes, identifiable only if enlarged.
4. And once again, place the piece
of paper in formalin.
Stomach
If you wish to inflate the stomach...
1.Fill your
syringe with your preservative and insert it into the duodenal
junction.
2. Inject the fixative as you hold
the needle in place with forceps.
3.On removing the syringe, some leakage
may occur but the majority of it will remain in the stomach.
Fixation and
Trimming Protocol for Stomach
Next Step Please
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Site designed, written,
and selected pictures illustrated by Erin Parsoneault, inspired by D.E.Devor-Henneman,
and edited/mentored byDr.
J.M.Ward