Authors:	Velikodvorskaya, TV; Volkov, IY; Vasilevko, VT; Zverlov, VT; Chekanovskaya, LV; Piruzian, ES
Title:	Construction and properties of a deletion variant of the laminarinase (Lam A) from Thermogota neapolitana and expression of the modified gene in protoplasts of Nicotiana plumbaginifolia
Source:	GENETIKA, 34: (5) 605-609 MAY 1998

Abstract:
Some properties of the deletion derivative of the gene encoding the beta-1,3-glucanase (laminarinase) from extremely thermophiIic bacteria Thermotoga neapolitana were studied. A high specific activity of the enzyme under normal conditions of plant growth was revealed. A hybrid gene containing the signal sequence of the carrot extensin gene and deletion derivative of the LamA L4 under control of the constitutive T-R2 promoter was transferred into plant cells. The functional activity of the carrot extensin gene signal sequence and the possibility of the detection of thermostable bacterial glucanase against the background of nonthermostable plant glucanases were shown.

Research Institutions:	Russian Acad Sci, Inst Mol Genet, Moscow 123182, Russia.
Publisher:	MEZHDUNARODNAYA KNIGA, 39 DIMITROVA UL., 113095 MOSCOW, RUSSIA