Authors:	Zverlov, VV; Volkov, IY; Lunina, NA; Velikodvorskaya, GA
Title:	Enzymes of thermophilic anaerobic bacteria hydrolyzing cellulose, xylan, and other beta-glucans
Source:	MOLECULAR BIOLOGY, 33: (1) 89-95 JAN-FEB 1999

Abstract:
Genes for 24 glycosylhydrolases of three thermophilic anaerobic eubacteria (Clostridium thermocellum, Anaerocellum thermophilum, and Thermotoga neapolitana) were cloned in Escherichia coli. The nucleotide sequences were determined for 13 genes, and the domain structures of the encoded proteins were deduced by analysis of predicted amino acid sequences. Multidomain organization was found for the cellobiohydrolase CbhA of C. thermocellum, xylanase XynA, cellulases CelD and CelA of A. thermophilum, laminarinase LamA and xylanase XynA of T. neapolitana. The genes for T. neapolitana enzymes hydrolyzing laminarin (lamA and bglB) and cellulose (celA and celB), as well as the A. thermophilum enzymes hydrolyzing cellulose and various glucans (celA and manA), were found to be clustered in the host chromosome.

Reprints:	ZVERLOV VV,RUSSIAN ACAD SCI,INST MOL GENET;KURCHATOV SQ;MOSCOW 123182, RUSSIA.
Research Institutions:	Russian Acad Sci, Inst Mol Genet, Moscow 123182, Russia.
Publisher:	PLENUM PUBL CORP, CONSULTANTS BUREAU, 233 SPRING ST, NEW YORK, NY 10013 USA