"I have had volumes of media up to 200 liters made with Gelrite as the gelling
agent. Cold dispensing can be accomplished if the medium is constantly
agitated (with the Gelrite kept in suspension). You may have to modify your
mixing process (i.e. adding a propeller(s) on your stirrer, adding a method
of recirculation via the pump, or to increase the pump capacity). You will also
not need to have prolonged pauses in your dispensing as this will lead to
settling in your hose, etc. Media gelling with use of 1.6 grams/liter occurred
with half-strength to full-strength MS salts.
-
Ray Gillis, Research Assistant Manager
Oglesby Plants International"
Gelrite 1.6 gr/l 35 45
-------------------------------------------
NH4NO3 1000 1500
KNO3 1500 2000
Ca(NO3)2.4H20 500 800
MgSO4.7H2O 250 400
KH2PO4 200 200
FeEDDHA 30 80
KJ 0.2
Micro Holland 2 ml
Vit Rosa 1 ml
CH 400
Inosit 100
Ade 20
Sucrose+Glucose 20 gr +5 gr
pH 5.7
H2O ad 1000 ml
Micro Holland–Mn Dicotyledone
------------------------------------------------------
H3BO3 800 8
MnSO4.1H2O 800 8
ZnNa2EDTA 800 8
Na2MoO4.2H2O 20 0.20
CuSO4.5H2O 5 0.05
CoCl2.6H2O 2 0.02
NiCl2.6H2O 1 0.01
H2SO4 0.1 M 1 ml
H2O ad 200 ml *2 ml
.
.
Micro Holland–Mn–B) Monootyledone [Bamboo]
------------------------------------------------------
H3BO3 300 3
MnSO4.1H2O 800 8
ZnNa2EDTA 800 8
Na2MoO4.2H2O 20 0.20
CuSO4.5H2O 10 0.10
CoCl2.6H2O 2 0.02
NiCl2.6H2O 1 0.01
H2SO4 0.1 M 1 ml
H2O ad 200 ml *2 ml
.
.
Vit Rosa Dicot
-----------------------------------------------------
Thiamine B1 100 1.0
Nicotinic acid NA 50 0.5
Pyridoxine B6 50 0.5
Glycine 200 2
Ca Pantothenate 200 2
Biotin, D(+) 2 0.02
Folic acid 50 0.5
H2O ad 100 *1 ml
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