Father: Prince Ercole II of Este-Ferrara
Born: May 4, 1508
Married: Unknown
Died: October 3, 1559 in Ferrara

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Mother: Princess Renee Duchess of Chartres
Born: October 25, 1510 in Blois
Died: June 12, 1575 in Montargis

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Children:
1. Princess Anna of Este (b.11/16/1531)
2. Prince Alfonso II of Este-Ferrara (b.11/28/1533)
3. Princess Eleonore of Este (b.6/19/1537)
4. Cardinal Luigi of Roman Catholic Church (b.12/25/1538)

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