San Jose, CA 95134

530-706-8208(voicemail/fax)

rereeves@excite.com

Scientist/ Research Associate with 7 years experience in biotechnology. Most recently involved in the design and development of bioluminescent transgenic animals and stable cell lines. Master of Arts degree (Biochemistry) from Boston University 1995.

To apply my molecular biology and physiology research experience as part of a program at an expanding biotechnology company.

• DNA/RNA molecular biology techniques (cloning, BAC cloning, site-directed mutagenesis, northern blotting, PCR and PCR primer design, RT-PCR,)

• Create, characterize and maintain luciferase-expressing stable cell lines

• Maintain tissue culture cell lines for ongoing transient transfection experiments

• Protein gel analysis (westerns, immunoblots)

• High throughput robotic programming and operation

• Gene overexpression, protein purification

• Small animal handling (mice, rats, and rabbits; anesthesia (isoflurane and ketamine), bioluminescent imaging, surgery prep and assist, blood collection)

• Histology (perfusion, tissue fixation, embedding, staining, serial sectioning)

• Radioisotope use and monitoring (P32, C14, S35, H3; bioassay, tracer analysis)

• RFLP analysis (mouse genome)

• Agarose and polyacrilamide gel electrophoresis

• Photography (processing, printing, darkroom equipment)

• Protocol design and authoring

• Lab support (grant preparation, chemical inventory, ordering)

• Fluent in both Windows and Macintosh operating systems

• Computer support (maintenance of software, hardware, network, training of lab personnel)

• Computer applications (DNA sequence analysis and primer design: VectorNTI; Statistics: StatView; Database management: FileMaker, 4D, MS Access; HTML/web page design and testing)

• Supervision and training of 3 undergraduates and post-graduate researchers in techniques and methodology

2001-2003 Research Associate II

Dept. of Light Producing Transgenic Animals, Xenogen Corporation, Alameda, CA (Dr. Scott Malstrom)

• Created 3 clones sent for microinjection

• Adapted and tested protocols for large-scale (>10 KB) BAC cloning

• Researched, ordered and performed serum assays and Westerns as part of a group multi-drug study/publication (in submission)

• Developed and applied high-throughput PCR screening technology for transgenic mouse genotyping.

• Created and maintained stable cell lines; measured luciferase expression in response to hormone stimulation

• Provided support and training for in-house personnel regarding use of IVIS™ camera system and associated software for secondary usages

• Provided input and data necessary for development of company manuals, training guides and planned hardware improvements

2000-2001 Research Scientist I

Versicor, Inc., Fremont, CA (Dr. Joaquim Trias)

• Cloned and overexpressed target genes as part of team characterizing novel antibacterial agents

• Assayed by HPLC in vivo-derived compounds for pharmokinetics studies

1996-2000 Research Assistant II

Dept. of Neurobiology, Physiology and Behavior, Univ. of California, Davis, CA (Dr. Barbara Horwitz)

• Developed and applied PCR screening technology for transgenic mouse construction

• Tracked behavioral effects of mutant mouse oxygen consumption and protein expression

1995-1996 Instructor

Experience Unlimited, Sacramento, CA

• Trained classes of 12 students in job skills

• Lead discussion groups and conducted mock interviews

1989-1995 Graduate Student, Research Assistant

Boston Univ., Cardiovascular Institute, Boston, MA (Dr. Peter Brecher)

• Measured changes in gene production and expression in response to drug-induced hypertension

• Performed surgical implantation of osmotic minipumps and tissue collection

1993-1995 Teaching Assistant

Boston Univ., Boston, MA

• Taught 3 sections of 12+ students, "Introduction to Biotechnology Techniques"

1988-1989 Lab Assistant

School of Medicine, Univ. of California, Davis, CA (Dr. Michael Holander)

• Cloned and tested transgenic yeast strains

Summer 1988 Lab Assistant

Dept. of Anatomy Univ. of California, San Francisco, CA (Dr. Charles Ordahl)

• Identified sequence elements involved with DNA binding in ) muscle development

• DNA sequencing, cloning

Summer 1987 Alumni Outreach Coordinator

Department of Gifts & Development, Univ. of California, Davis, CA

• Directed group of 35 for phone survey and alumni solicitation

"Multi-drug study paper" Scott Malstrom, others. In submission

"Adiposity and serum leptin increase in fatty (fa/fa) BNZ neonates without decreased VMH serotonergic activity" Horwitz BA, Hamilton JS, Routh VH, Green K, Havel P, Chan A

AMERICAN JOURNAL OF PHYSIOLOGY-ENDOCRINOLOGY AND METABOLISM

37: (6) E1009-E1017 JUN 1998

"Effects of age, gender, and senescence on -adrenergic responses of isolated F344 rat brown adipocytes in vitro" Annette M. Gabaldón, Roger B. McDonald, and Barbara A. Horwitz

AMERICAN JOURNAL OF PHYSIOLOGY-ENDOCRINOLOGY AND METABOLISM

37: (4) E726-E736 APR 1998