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Now, I know I may have lost some of you
there, but it's really quite simple. Look at these results
as an example. The DNA that was immobilized on the nylon is
the target and the single-stranded radioactive DNA is the
probe. When the two are compatible, they anneal to one
another or fit together like a zipper closing. Then the
nylon membrane is exposed to film and the places where the
"hot" probe is bound emit bursts of light as the
radioactivity decays, leaving a signal on the x-ray film.
Voila!
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