| Protein isolation and purification using conventional procedures. HPLC, FPLC and other techniques of protein characterization. Electrophoresis, 2 dimensional electrophoresis, Isoelectricfocussing and Western blotting. Analytical ultracentrifugation. EM analysis of protein fibrils. Small Angel X-ray Scattering (SAXS) of Proteins. Preliminary crystallization of proteins. UV, fluorescence, CD spectroscopy. Fluorometric studies by Fluorskan Multi well plate readers. Metal (Copper) binding to proteins by EPR. Characterization of secondary structure of proteins by ATR-FTIR. Characterization of protein conformations and H/D exchange by Mass Spectrometry. Dynamic light scattering and preliminary knowledge of NMR spectroscopy. Isothermal Titration Calorimetry and Differential Scanning Calorimetry Enzyme kinetic studies, enzyme assays, chemical modification/labeling of proteins and enzymes and proteolytic/chemical cleavage of proteins. DNA/RNA isolation and purification. Agarose gel electrophoresis and gel documentation. Site directed mutagenesis Solid phase peptide synthesis. Hanging drop method of crystal production. Mounting of crystal and collection of diffraction data. |
| Proteins: Spectroscopy: Calorimetry: Enzymology: DNA & RNA: Peptide Synthesis Crystallography: |
| Research Experience |