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Title |
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Quantitative Morphometric And Histochemical Studies Of Hair Follicles In Alopecia Areata |
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BY |
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Wadad Z. Moustafa*, Bassem S. Kotb**, Manal A. Bosseila*, Tarek A. Emara* …..
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*Department of Dermatology, Faculty of Medicine – Cairo University and **Department of Histology, Faculty of Medicine – Al Azhar University (Assuit)
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ABSTRACT |
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Alopecia areata (localized hair loss) is a common hair follicle disease with unclear pathogenesis. The aim of this work is to clarify the changes in the hair follicles that may occur during alopecia areata using image analysis. The study was conducted on 20 patients with alopecia areata and 5 healthy volunteers. Biopsies were taken after consent, for quantitative histological morphometric measurements and for histochemical analysis of hair follicles. The study revealed that there was a statistically significant decrease in the mean percent of anagen hair follicles and statistically significant increase in the mean percent of catagen hair follicles in 4mm punch biopsies taken from alopecia patients in comparison to that of normal volunteer. The study showed that there were statistically significant decreases in the following; anagenic and telogenic hair follicles areas, telogenic hair follicles perimeter, anagenic outer sheath thickness, hair follicles irregularity (catagen and telogen), and telogenic hair (diameter, area and perimeter). Also there were statistically significant increases in the following; hair follicles irregularities (anagen and telogen), and anagenic hair perimeter. Regards histochemical studies, there was a statistically significant decrease in the DNA staining affinity of hair follicles of anagen and catagen. The study concluded that the morphometric quantitative study of tangential histological sections of scalp biopsies with the aid of image analyzer is a feasible and easily technique for differentiation between of alopecia areata and normal hairs and their subtypes.
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