Antimicrobial Activities of Several Parts of Pinus brutia, Juniperus oxycedrus, Abies cilicia, Cedrus libani, and Pinus nigra

Metin Dığrak* Ahmet İlçim

Department of Biology, Faculty of Arts and Science, Kahramanmaraş Sütçü Imam University, 46045- Kahramanmaraş, TURKEY

M. Hakkı Alma

Department of Industrial Eng. of Forestry, Kahramanmaraş Sütçü Imam University Faculty of Forestry, 46100-Kahramanmaraþ, TURKEY

In this study, the antimicrobial activities of several parts of various trees grown in Kahramanmaraþ region (Turkey) were investigated by using disc diffusion method. Chloroform, acetone and methanol extracts of leaves, resins, barks, cones, and fruits of Pinus brutia Ten., Juniperus oxycedrus L., Abies cilicia Ant.& Kotschy Carr., Cedrus libani A. Rich. and Pinus nigra Arn. were prepared and tested against Bacillus megaterium DSM 32, Bacillus subtilis IMG 22, Bacillus cereus FMC 19, Escherichia coli DM Klebsiella pneumoniae FMC 3, Enterobacter aerogenes CCM 2531, Staphylococcus aureus Cowan 1, Mycobacterium smegmatis RUT, Proteus vulgaris FMC 1, Listeria monocytogenes Scoot A, Pseudomonas aeruginosa DSM 5007, Candida albicans CCM 314, Candida tropicalis MDC 86, and Penicillium italicum K.

The results showed that antifungal effects were not observed for the whole extracts E. coli could not be inhibited by any of the plant extracts except the chloroform and acetone extracts of the leaves of A. cilicia, which showed inhibition zones of 16-18 mm, respectively. All the plant extracts used in this study inhibited the development of the other bacteria studied. When compared the results found in this study to ampicillin standard, it was determined that microorganisms studied were, generally, susceptible, intermediate and resistant to the extracts of species when compared to ampicillin standard. On the other hand, acetone and methanol extracts of Juniperus fruits were found to be quite resistant.

Keywords: Pinus brutia, Juniperus oxycedrus, Abies cilicia, Cedrus libani, Pinus nigra, antimicrobial activity