| Name | Rank | Age | Date Entering Service | Period of Service | Remarks |
| Thomas Kilby Smith | Colonel | 32 | Oct. 31, 1861 | 3 yrs. | Promoted to Brig. General Aug. 11, 1863. |
| James A. Farden | Lt. Col> | 28 | Oct. 31, 1861 | 3 yrs. | Resigned Dec. 11, 1862 at Memphis, Tenn. |
| Cyrus W. Fisher | do | 34 | Oct. 31, 1861 | 3 yrs. | Promoted from Major Nov. 27, 1862; discharged Sept. 29, 1863. |
| Robert Williams | do | 21 | Sept. 11, 1861 | 3 yrs. | Promoted to Major from Captain Co. B Nov. 13, 1863; to Lt. Colonel Sept. 29, 1863; discharged Sept. 14, 1864. |
| Israel T. Moore | do | 30 | Sept. 10, 1861 | 3 yrs. | Promoted to Major from Captain Co. D Nov. 13, 1863; to Lt. Colonel Oct. 12, 1864; mustered out with regiment Aug. 15, 1865. |
| George F. Kili | Major | 25 | Sept. 5, 1861 | 3 yrs. | Promoted from Captain Co. K October 12, 1864; mustered out with regiment Aug. 15, 1865. |
| Columbus P. Brent | Surgeon | 30 | Sept. 27, 1861 | 3 yrs. | Mustered out Jan. 3, 1865 on expiration of term of service. |
| James Baggs | do | 25 | Aug. 28, 1861 | 3 yrs. | Promoted from Asst. Surgeon Feb. 10, 1865; mustered out with regiment Aug. 15, 1865. |
| Thomas L. Harper | Asst. Surgeon | 42 | Oct. 9, 1861 | 3 yrs. | Resigned Aug. 1, 1863. |
| James C. McCoy | Adjutant | 28 | Sept. 28, 1861 | 3 yrs. | Promoted to Captain Co. G Jan. 13, 1863 |
| Luther W. Saxton | do | 19 | Dec. 1, 1861 | 3 yrs. | Appointed from 1st Lieutenant Co. K Jan. 1, 1863; promoted to Captain, Co. A Nov. 27, 1863. |
| David A. Rees | do | 19 | Sept. 11, 1861 | 3 yrs. | Appointed from 1st Lieutenant Co. B Jan. 24, 1864; killed June 27, 1864, in battle of Kenesaw Mtn., Ga.; veteran. |
| William H. Hunt | do | 23 | Mch. 10, 1864 | 3 yrs. | Appointed from 1st Lietenant Co. G Aug. 2, 1864; transferred to Co. H Oct. 1, 1864. |
| John F. Cutter | do | 21 | Jan. 4, 1862 | 3 yrs. | Appointed from 1st Lietenant Co. H Oct. 1, 1864; mustered out Jan. 4, 1865, on expiration of term of service. |
| Edmund B. Updegrove | do | 20 | Sept. 11, 1861 | 3 yrs. | Appointed from 1st Lieutenant Co. B Jan. 4, 1865; promoted to Captain, Co. A May 3, 1865. |
| George W. Wilson | do | 19 | Dec. 12, 1861 | 3 yrs. | Promoted to Com. Sergeant from Sergeant Co. B Oct. 19, 1864; to 1st Lieutenant and Adjutant May 31, 1865; mustered out with regiment Aug. 31, 1865; veteran. |
| Benjamin W. Good | R.Q.M. | 35 | Sept. 12, 1861 | 3 yrs. | Resigned May 1, 1862 |
| Henry B. Whetsel | do | 34 | Sept. 10, 1861 | 3 yrs. | Promoted to Q.M. Sergeant from private Co. I Sept. 10, 1861; to 2nd Lieutenant and Asst. R.Q.M. May 13, 1862; 1st Lieutenant and R.Q.M. March 16, 1863; promoted to Captain and Asst. Quartermaster -. |
| John G. Hauck | do | 18 | Jan. 20, 1862 | 3 yrs. | Promoted to Q.M. Sergeant from Sergeant Co. H Jan. 22, 1864; to 2nd Lieutenant Co. - Aug. 26, 1864; 1st Lieutenant and R.Q.M. Oct. 12, 1864; Mustered out with regiment Aug. 31, 1865; veteran. |
| Joseph Morris | Chaplain | 54 | Feb. 16, 1862 | 3 yrs. | Resigned Nov. 19, 1863 |
| John H. Enoch | Sgt. Maj. | 20 | Sept. 13, 1861 | 3 yrs. | Promoted to 2nd Lieutenant Co. C Nov. 1, 1862. |
| Peter Gosnel | do | 25 | Sept. 16, 1861 | 3 yrs. | Promoted from Sergeant Co. B April 15, 1863; to Captain U.S. Colored Troops Aug. 10, 1863. |
| Miles W. Eliott | do | 20 | Sept. 11, 1861 | 3 yrs. | Promoted from Sergeant Co. B Feb 27, 1864; to 1st Lieutenant Co. H Feb. 14, 1865. |
| James Clark | do | 22 | Nov. 26, 1861 | 3 yrs. | Promoted from 1st Sergeant Co. K Jan. 1, 1864; died Feb. 18, 1864; veteran. |
| Charles W. Craig | do | 19 | Sept. 7, 1861 | 3 yrs. | Promoted from Sergeant Co. C March 31, 1865; mustered out with regiment Aug. 15, 1865. |
| Charles R. Ashmead | Q.M.S. | 25 | Sept. 5, 1861 | 3 yrs. | Promoted from Sergeant Co. E May 1, 1862; to 2nd Lieutenant Co. I Nov. 1, 1862. |
| Foster Black | do | 25 | Dec. 2, 1861 | 3 yrs. | Promoted from private Co. E April 6, 1863; discharged Jan. 16, 1864, at Larkinsville, Ala. on Surgeon's certificate of disability. |
| David B. Hedrick | do | ... | Sept. 5, 1861 | 3 yrs. | Promoted from Sergeant Co. A Nov. 1, 1864; reduced to ranks and transferred to Co. A June 18, 1865. |
| Thomas C. Bennett | do | 21 | Dec. 12, 1861 | 3 yrs. | Promoted from Sergeant Co. B June 18, 1865; mustered out with regiment Aug. 15, 1865; veteran. |
| George E. Mullen | Com. Ser. | 27 | Sept. 1, 1861 | 3 yrs. | Reduced to ranks and transferred to Co. C July 18, 1863. |
| James P. Wiatt | do | 28 | Sept. 11, 1861 | 3 yrs. | Promoted from Sergeant Co. C July 18, 1863; promoted to 1st Lieutenant Co. D Oct. 12, 1864; veteran. |
| Peter G. Dalzell | do | 18 | Oct. 20, 1861 | 3 yrs. | Promoted from Sergeant Co. D June 18, 1865; mustered out with regiment Aug. 15, 1865; veteran. |
| Benj. P. Haggett | Hos. St'd | 40 | Nov. 18, 1861 | 3 yrs. | Promoted from private Co. H -, 1861; discharged Dec. 20, 1862 at Memphis, Tenn., on Surgeon's certificate of disability. |
| Charles Bassett | do | 41 | Sept. 10, 1861 | 3 yrs. | Promoted from private Co. D Sept. 3, 1862; mustered out Sept. 29, 1864, at East Point, Ga., on expiration of term of service. |
| James Lemon | do | 22 | Feb. 2, 1864 | 3 yrs. | Promoted from private Co. H Oct. 1, 1864; mustered out with regiment Aug. 15, 1865. |
| Leonard W. Brown | Prin. Mus | 22 | Oct. 27, 1861 | 3 yrs. | Promoted from musician Co. B June 5, 1862; reduced to musician and transferred to Co. B -. |
| Thomas Mullen | do | 22 | Sept. 5, 1861 | 3 yrs. | Promoted from Musician Co. F Jan. 22, 1864; reduced to ranks and transferred to Co. F June 21, 1865. |
| Joseph Utz | do | 25 | Sept. 5, 1861 | 3 yrs. | Promoted from musician Co. A Jan. 22, 1864; mustered out with regiment Aug. 15, 1865. |