ACROSS | DOWN |
3 Vectors are used to insert library DNA into these host organisms | 1 One thing a cDNA library lacks |
4 Another thing a cDNA library lacks | 2 Easiest target for affinity chromatography to collect mRNA |
9 Run RNA on a gel, transfer to nitrocellulose or nylon, add labeled ssDNA probe to find the desired RNA (2 wds) | 5 What makes cDNA into dsDNA for insertion into a host (2 wds) |
10 Chop up DNA, run it on a gel, transfer to nitrocellulose or nylon, add labeled ssDNA probe to find the desired DNA (2 wds) | 6 A library containing reverse transcripts of the mRNA found in target cells |
7 One way to probe a DNA library to find a desired sequence | |
8 A library containing fragments of all the DNA in an organism's genome |