DNA Libraries Brought to you by the Cogix Crossword Wizard

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ACROSS DOWN
3 Vectors are used to insert library DNA into these host organisms 1 One thing a cDNA library lacks
4 Another thing a cDNA library lacks 2 Easiest target for affinity chromatography to collect mRNA
9 Run RNA on a gel, transfer to nitrocellulose or nylon, add labeled ssDNA probe to find the desired RNA (2 wds) 5 What makes cDNA into dsDNA for insertion into a host (2 wds)
10 Chop up DNA, run it on a gel, transfer to nitrocellulose or nylon, add labeled ssDNA probe to find the desired DNA (2 wds) 6 A library containing reverse transcripts of the mRNA found in target cells
7 One way to probe a DNA library to find a desired sequence
8 A library containing fragments of all the DNA in an organism's genome



Based on lecture notes taken in BSCI 420: Cell Biology Lectures under Dr. S. Wolniak, University of Maryland College Park.

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