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3 Uses simultaneous DNA polymerase reactions followed by four-lane gel or detector-assisted capillary electrophoresis to produce a DNA sequence (2 wds) | 1 The key ingredient to Sanger sequencing, they cause the polymerase reaction to stop at random incidences of specific bases |
4 This reaction runs DNA template through a series of thermal cycles with DNA polymerase, DNA primers, dNTPs and magnesium to amplify desired DNA | 2 Cooling split dsDNA srands to make them come back together |
5 Splitting dsDNA by heat | 6 This polymerase from Thermus aquaticus is commonly used because of its high temperature stability |