ACROSS | DOWN |
3 Vesicles from here will probably be the top layer | 1 Step 4 (80-100000g at 60 min) leaves these in the supernatant |
7 Resuspending the step four pellet and centrifuging it on a glucose density gradient allows this | 2 Step 3 (20000g at 20 min) pellets these |
9 This comes apart at 4 deg C and assembles at 45 deg C, allowing cyclic centrifugal purification | 4 The pellet at the end of Step 4 (80-100000g at 60 min) is also called this |
10 The pellet at the end of Step 4 contains these from the Golgi, ER, lysosomes | 5 Step 1 (1000g at 10 min) pellets these (2 words) |
11 Vesicles from here will probably be the bottom layer due to what's attached to them | 6 Step 4 (80-100000g at 60 min) also leaves these in the supernatant |
8 Step 2 (5000g at 20 min) pellets these |