Velocity Centrifugation or What's in the Pellet? Brought to you by the Cogix Crossword Wizard

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ACROSS DOWN
3 Vesicles from here will probably be the top layer 1 Step 4 (80-100000g at 60 min) leaves these in the supernatant
7 Resuspending the step four pellet and centrifuging it on a glucose density gradient allows this 2 Step 3 (20000g at 20 min) pellets these
9 This comes apart at 4 deg C and assembles at 45 deg C, allowing cyclic centrifugal purification 4 The pellet at the end of Step 4 (80-100000g at 60 min) is also called this
10 The pellet at the end of Step 4 contains these from the Golgi, ER, lysosomes 5 Step 1 (1000g at 10 min) pellets these (2 words)
11 Vesicles from here will probably be the bottom layer due to what's attached to them 6 Step 4 (80-100000g at 60 min) also leaves these in the supernatant
8 Step 2 (5000g at 20 min) pellets these



Based on lecture notes taken in BSCI 420: Cell Biology Lectures under Dr. S. Wolniak, University of Maryland College Park.

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