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Signal Transduction by Polypeptide Growth Factors
Weizmann Institute of Science, Rehovot, Israel
Organizers: Yosef Yarden & Johannes L. Bos, Professors
September 6-18, 1998
Transient Transfection Techniques (DEAE Dextran, Calciumphosphate, Electroporation, Lipofectamine)
Ligand Binding (Ligand Iodination, Receptor Binding Assay, Receptor Down-Regulation)
Immunoprecipitation
Western Blotting
Purification of GST-Fusion Proteins
GTPase Fish Assay
Kinase Assays (Immunocomplex and In-Gel Kinase Assays, Mini Column Assay)
Reporter Gene Assays (Luciferase, b-Galactosidase)
Electrophoresis Mobility Shift Assays
Yeast-Two-Hybrid System
Immunofluorescence
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