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Standard Molecular Biology Techniques
PCR/ RT-PCR Techniques
Cell Culture Techniques (CCL39, COS, CHO cells)
Transient and Stable Transfection Methods (DEAE-Dextran, Electroporation)
Reporter Gene Assay (b-Galactosidase/ Staining of Cells)
Northern Blot Analysis
Southern Blot Detection
Western Blot Analysis
Radioligand Binding Assays (Saturation, Competition)
[35S]GTP gS Binding Assays
Direct and Indirect Measurements of Adenylate Cyclase Activities (cAMP Accumulation)
Measurements of Phospholipase C Activities (Phosphoinositide Turnover/ total [3H]-IPx accumulation)
Measurements of Intracellular Calcium (Cells)
In situ-Hybridization
Statistical Analysis
Software: Word, Excel, Origin, SigmaPlot, GraphPad Prism, Ligand, PrimerDesigner, PowerPoint, TopCount Software, FLIPR-TM II Statistical Software
Initial introduction of molecular biology equipment to the laboratory
Teaching of labsratory techniques
Supervising of Students
Introduction of new protocols and new assays to the laboratory (SPA-based techniques, set-up for FLIPR etc.), which are now being used for high throughput screenings
World-wide collaborations with research laboratories
Initiation and organisation of weekly seminar to group Novartis laboratory heads, post-docs and graduate students from different research areas and research departments
Oral presentations in organized Novartis seminar and at the ETH Zurich
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