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Standard Molecular Biology Techniques
Expression and Purification of GST-fusion proteins
PCR Techniques
Cell Culture of adherent and suspension cells (HEK293, CHO, COS, HeLa, NIH3T3, Swiss3T3, R777, Jurkat T)
Transient and Stable Transfection Techniques (Superfect, Lipofectamine, FuGENE, Calcium-Phosphate, Electroporation, Flp-In)
Reporter Gene Assays (Luciferase, b-Galactosidase etc.)
Experience with numerous Cell Signalling Inhibitors
Electromobility Shift Assays
Western Blot Analysis
Immunoprecipitation
Kinase Assays (MAPK, IkB kinases, PAK)
Rho-GTP and Rac-GTP pull-down Assays
Software: Word, Excel, Cricketgraph, Kaleidagraph, Canvas, DNA Strider, BLAST, AdobePhotoshop, PowerPoint,IQMac Phosphorimager Quantitative Analysis
Programmimg Skills (HTML)
Collaborations with numerous research laboratories
Teaching of laboratory techniques
Project design for graduate students
Lab management
Oral presentations in laboratory and departmental meetings
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