72h Microbiotest
For Toxicity Screening of
Pure Compounds - Effluents - Sediments -
Surface and Ground Waters - Wastewaters
Each ALGALTOXKIT F contains all the materials necessary to perform two
growth inhibition tests with the freshwater microalgae Selenastrum capricornutum.
Easy to follow instructions and detailed illustrations are provided in
the kits for the conduct of range-finding and definitive tests. The test
organisms are included in the kits in " algal beads" from which
the microalgae can easily be set free on demand, in less than 30 min,
to supply the live biota for the conduct of the assays.
Test criterion
· The ALGALTOXKIT F is a 72h assay based on growth inhibition of
the micro-algae, with calculation of the 72hEC50.
Reproducibility
· Algal beads of high quality produced in strictly controlled conditions
preclude variability associated with recruitment/ maintenance of live
stocks of microalgae in exponential growth phase, in conventional bioassays.
· Highly uniform quality of the test medium is achieved by simple
dilution of concentra-ted solutions of selected chemicals, with deionized
water.
· Standardized "long cell" test containers constructed
of biologically inert materials ensure uniform exposure conditions.
· A Quality Control Test with a reference chemical is described
in detail, for accuracy and reproducibility check.
Cost-Effectiveness
· The ALGALTOXKIT makes use of unique disposable "long cell"
exposure chambers, which also serve as measuring cuvettes for direct determination
of algal growth by optical density measurements.
· Labor time for testing is significantly reduced by the direct
measurement of algal growth in the long cells in a colorimeter of spectrophotometer.
· Shelf-life of the algal beads is guaranteed for several months
when stored properly, reducing test scheduling constraints.
Contents
· Tubes with algal beads, concentrated algal growth and toxicant
dilution medium, long cells and holding trays.
· Detailed Standard Operational Procedure brochure, abbreviated
Bench Protocol, OD/N regression sheet, data scoring sheets and graphical
EC50 calculation sheets.
· Specification sheet with batch number of the algal beads and
the media.
User-Friendliness
· Bioassays can be started within 30 min. from the "de-immobilization"
of the microalgae from the beads
· Simple handlings and scorings.
· Daily scoring of the optical density of the microalgae in the
long cells takes less than 15 min.
· A floppy disc for easy Toxkit data treatment can be obtained
on demand.
Sensitivity
· Comparable to the sensitivity of the algal growth inhibition
test with microalgae from laboratory cultures..
Validation
· The ALGALTTOXKIT is used in many laboratories worldwide for routine
screening of chemicals and environmental samples.
· Substantial number of publications reporting data for various
uses.
· The ALGALTOXKIT microbiotest strictly adheres to the protocols
for regulatory testing with microalgae, prescribed by international organizations
such as e.g. the OECD and the ISO.
A list of selected references is available upon request.
N.B. All
materials included in the
ALGALTOXKIT are also made
available separately.
ALGALTOXKIT
F is a registered trademark of Creasel Ltd, Deinze, Belgium.
