24h Microbiotest
For Toxicity Screening of
Pure Compounds - Contaminated Seawater and estuarine/marine Sediments
- Effluents/Wastes released in Estuaries or coastal Waters - Biotoxins
Each ARTOXKIT M contains all the materials necessary to perform six acute toxicity tests with the marine crustacean Artemia salina (renamed Artemia franciscana). Easy to follow instructions and detailed illustrations are provided in the kits for the conduct of range-finding and definitive tests. The test organisms are included in the kits as " dormant eggs (cysts)" which can easily be hatched on demand, in less than 24h, to supply the live biota for the conduct of the assays.
Test criterion
· The ARTOXKIT M is a 24h assay based on mortality of the test
organisms, with calculation of the 24h LC50.
Reproducibility
· Cysts of high quality produced in strictly controlled conditions
preclude variability associated with recruitment/ maintenance of live
stocks in conventional bioassays.
· Highly uniform quality of the test medium is achieved by simple
dilution of concentrated solutions of selected salts with deionized water.
· Standardized microplate test containers constructed of biologically
inert materials ensure uniform exposure conditions.
· A Quality Control Test with a reference chemical is described
in detail, for accuracy and reproducibility check.
Cost-Effectiveness
· Cysts can be hatched on demand, eliminating the need and the
costs of continuous culturing and maintenance of test organisms.
· Minimal equipment needed for test performance: dissecting microscope
- small incubator - conventional laboratory glass-ware.
· Shelf-life of cysts guaranteed for several months when stored
properly, reducing test scheduling constraints.
Contents
· Tubes with cysts, concentrated hatching and toxicant dilution
medium, hatching and test containers and micropipettes for the transfer
of the organisms.
· Detailed Standard Operational Procedure brochure, abbreviated
Bench Protocol, data scoring sheets and graphical LC50 calculation sheets.
· Specification sheet with batch number of the cysts and the media.
User-Friendliness
· Simple handlings and scorings.
· Total performance time of the assay approximately 1 hour.
· A floppy disc for easy Toxkit data treatment can be obtained
on demand.
Sensitivity
· Comparable to that of various other estuarine/marine biota.
· Specifically sensitive to biotoxins produced by freshwater and
marine micro-algae.
Applications
· One of the most euryhaline test species. which allows to perform
bioassays on waters in a salinity ranging from 10 ppt (brackish water)
to 35 ppt (seawater)
· Acute microbiotest with Artemia salina used for regulatory marine
toxicity testing in several countries.
A list of selected references is available upon request.
N.B. All
materials included in the
ARTOXKIT are also made
available separately.
ARTOXKIT
M is a registered trademark of Creasel Ltd, Deinze, Belgium.
