48h Microbiotests
For Toxicity Screening of
Pure Compounds - Effluents - Sediments -
Surface and Ground Waters - Wastewaters
The DAPHTOXKITS F magna and DAPHTOXKIT F pulex contain all the materials necessary to perform six acute toxicity tests with the freshwater crustaceans Daphnia magna and Daphnia pulex respectively. Easy to follow instructions and detailed illustrations are provided in the kits for the conduct of range-finding and definitive tests. The test organisms are included in the kits as " dormant eggs (ephippia)" which can easily be hatched on demand in 72h, to supply the live biota for the conduct of the assays.
Test criterion
· The DAPHTOXKIT F microbiotests are 48h assays based on immobility
or mortality of the test organisms, with calculation of the EC50 or LC50.
Reproducibility
· Ephippia of high quality produced in strictly controlled conditions
preclude variability associated with recruitment/ maintenance of live
stocks in conventional bioassays.
· Highly uniform quality of the test medium is achieved by simple
dilution of concen-trated solutions of selected salts with deionized water.
· Standardized microplate test containers constructed of biologically
inert materials ensure uniform exposure conditions.
· A Quality Control Test with a reference chemical is described
in detail, for accuracy and reproducibility check.
Cost-Effectiveness
· Ephippia can be hatched on demand, eliminating the need and the
costs of continuous culturing and maintenance of test organisms.
· Minimal equipment needed for test performance: light table or
dissecting microscope - small incubator - conventional laboratory glassware.
· Shelf-life of ephippia guaranteed for several months when stored
properly, reducing test scheduling constraints.
Contents
· Tubes with ephippia, concentrated hatching and toxicant dilution
medium, hatching and test containers and micropipettes for the transfer
of the organisms.
· Detailed Standard Operational Procedure brochure, abbreviated
Bench Protocol, data scoring sheets and graphical L(E)C50 calculation
sheets.
· Specification sheet with batch number of the ephippia and the
media.
User-Friendliness
· Simple handlings and scorings.
· Total performance time of the assay approximately 1 hour.
· A floppy disc for easy Toxkit data treatment can be obtained
on demand.
Sensitivity
· Comparable to the sensitivity of Daphnids from laboratory cultures.
Validation
· DAPTHOXKITS are used in many labora-tories wordlwide for routine
screening of chemicals and environmental samples.
· Substantial number of publications reporting data for various
uses.
· The acute DAPHTOXKIT microbiotests strictly adhere to the protocols
for regulatory testing with Daphnias prescribed by international organi-zations
such as e.g. the OECD and the ISO..
A list of selected references is available upon request.
N.B. All
materials included in the
DAPHTOXKITS are also made
available separately.
DAPHTOXKIT
F magna and DAPHTOXKIT F pulex are registered trademarks of Creasel Ltd,
Deinze, Belgium.
