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Living logic
GM bugs that do the same job as the components of a microchip are revealed in the US 

Exclusive from New Scientist magazine 


Genetically engineered bugs that do the same job as the components of a microchip have been created by a US team.

These smart bugs will crunch on chemical inputs rather than digital bits. They could one day be sent into waste-water plants to hunt out toxic chemicals. Or they could tell doctors what proteins are present in body fluids.


 
  
Just like a silicon-based AND gate that requires two electrical inputs, the bacterial logic gate will only produce an output when both its chemical inputs are present. It is the combination of such simple functions that allows computers to perform highly complex tasks, says Michael Simpson, a physicist at the Oak Ridge National Laboratory in Tennessee.

But PCs with living logic circuits are still a long way off, warns Tim Gardner, a bioengineer and president of Cellicon Biotechnologies in Boston. For one thing, the basic components are not as good as silicon. "They are slower, noisier and more difficult to manipulate," he says.


Complex gates 


Nature already combines simple functions to good effect, says Simpson. "When you look at genetic circuits you see things that look like binary logic and feedback patterns." The challenge is to manipulate them to your advantage.

So Simpson, together with Gary Sayler and James Fleming, modified Pseudomonas putida cells to produce both AND and OR gates (see Graphic). They are now trying to produce more complex gates. The trick, he says, is to choose the genes to suit the kind of inputs and outputs you want.

In the case of the AND gate, for example, they used chemical "inducers" as inputs. One causes a gene to make a protein that the second input inducer must have to express the output enzyme. You need both inducers to produce an output, which could, for example, be a bioluminescent enzyme. 


Normal metabolism 


Simpson is optimistic about the future of bug-based chips. "We're trying to take advantage of what cells do well - processing information on the molecular scale," he says. 

With the right sequence of inputs and outputs, you could have a number of gates inside a single cell, with the output of one gate becoming an input of another. In theory, a single cell could produce massively parallel complex functions. 

Because the chemical inputs don't alter the normal control metabolism, the cells can still reproduce and survive. But in a competitive environment with other unmodified cells they would be at a disadvantage, says Simpson, as they would be expending energy on computations instead of survival.

Correspondence about this story should be directed to letters@newscientist.com 

1900 GMT, 23 May 2001

Duncan Graham-Rowe 
New Scientist Online News

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