This is the full sequence of a protein called EGFP (Enhanced
Green Fluorescent Protein)
(see: Roger Y. Tsien, Annu. Rev. Biochemistry
(1989) 67).
I am currently looking for mutations of this protein
optimising its utility as an optical sensor in cell biology.
Met
|
Val
|
Ser
|
Lys
|
Gly
|
Glu
|
Glu
|
Leu
|
Phe
|
Thr
|
Gly
|
Val
|
Val
|
Pro
|
Ile
|
Leu
|
Val
|
Glu
|
Leu
|
Asp
|
Gly
|
Asp
|
Val
|
Asn
|
Gly
|
His
|
1
|
1a
|
|
|
|
5
|
|
|
|
|
10
|
|
|
|
|
15
|
|
|
|
|
20
|
|
|
|
|
25
|
|
Lys |
Phe |
Ser |
Val |
Ser |
Gly |
Glu |
Gly |
Glu |
Gly |
Asp |
Ala |
Thr |
Tyr |
Gly |
Lys |
Leu |
Thr |
Leu |
Lys |
Phe |
Ile |
Cys |
Thr |
Thr |
|
26
|
|
|
|
30
|
|
|
|
|
35
|
|
|
|
|
40
|
|
|
|
|
45
|
|
|
|
|
50
|
|
Gly |
Lys |
Leu |
Pro |
Val |
Pro |
Trp |
Pro |
Thr |
Leu |
Val |
Thr |
Thr |
Leu |
Thr |
Tyr |
Gly |
Val |
Gln |
Cys |
Phe |
Ser |
Arg |
Tyr |
Pro |
|
51
|
|
|
|
55
|
|
|
|
|
60
|
|
|
|
|
65
|
|
|
|
|
70
|
|
|
|
|
75
|
|
Asp |
His |
Met |
Lys |
Gln |
His |
Asp |
Phe |
Phe |
Lys |
Ser |
Ala |
Met |
Pro |
Glu |
Gly |
Tyr |
Val |
Gln |
Glu |
Arg |
Thr |
Ile |
Phe |
Phe |
|
76 |
|
|
|
80 |
|
|
|
|
85 |
|
|
|
|
90 |
|
|
|
|
95 |
|
|
|
|
100 |
|
Lys |
Asp |
Asp |
Gly |
Asn |
Tyr |
Lys |
Thr |
Arg |
Ala |
Glu |
Val |
Lys |
Phe |
Glu |
Gly |
Asp |
Thr |
Leu |
Val |
Asn |
Arg |
Ile |
Glu |
Leu |
|
101 |
|
|
|
105 |
|
|
|
|
110 |
|
|
|
|
115 |
|
|
|
|
120 |
|
|
|
|
125 |
|
Lys |
Gly |
Ile |
Asp |
Phe |
Lys |
Glu |
Asp |
Gly |
Asn |
Ile |
Leu |
Gly |
His |
Lys |
Leu |
Gly |
Tyr |
Asn |
Tyr |
Asn |
Ser |
His |
Asn |
Val |
|
126 |
|
|
|
130 |
|
|
|
|
135 |
|
|
|
|
140 |
|
|
|
|
145 |
|
|
|
|
150 |
|
Tyr |
Ile |
Met |
Ala |
Asp |
Lys |
Gln |
Lys |
Asn |
Gly |
Ile |
Lys |
Val |
Asn |
Phe |
Lys |
Ile |
Arg |
His |
Asn |
Ile |
Glu |
Asp |
Gly |
Ser |
|
151 |
|
|
|
155 |
|
|
|
|
160 |
|
|
|
|
165 |
|
|
|
|
170 |
|
|
|
|
175 |
|
Val |
Gln |
Leu |
Ala |
Asp |
His |
Tyr |
Gln |
Gln |
Asn |
Thr |
Pro |
Ile |
Gly |
Asp |
Gly |
Pro |
Val |
Leu |
Leu |
Pro |
Asp |
Asn |
His |
Tyr |
|
176 |
|
|
|
180 |
|
|
|
|
185 |
|
|
|
|
190 |
|
|
|
|
195 |
|
|
|
|
200 |
|
Leu |
Ser |
Thr |
Gln |
Ser |
Ala |
Leu |
Ser |
Lys |
Asp |
Pro |
Asn |
Glu |
Lys |
Arg |
Asp |
His |
Met |
Val |
Leu |
Leu |
Glu |
Phe |
Val |
Thr |
|
201 |
|
|
|
205 |
|
|
|
|
210 |
|
|
|
|
215 |
|
|
|
|
220 |
|
|
|
|
225 |
|
Ala |
Ala |
Gly |
Ile |
Thr |
Leu |
Gly |
Met |
Asp |
Glu |
Leu |
Tyr |
Lys |
|
|
|
|
|
|
|
|
|
|
|
|
|
226 |
|
|
|
230 |
|
|
|
|
235 |
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
back