Bienvenvue au CSSA en Fribourg Homepage

Chinese Scholars and Students Association in Friborug, Switzerland

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Bienvenvue au CSSAF Homepage. CSSAF est une organisation pour tous les Chinois ( Etudiant(e)s ou Erudites) qui etudie ,travaille ou vit en Fribourg. Elle fournit a tous les Chinois en Fribourg toutes facilites si possible pour etudier,rechercher ou vivre. Tous notre membres travaillerons emsemblement avec le plus grande activites. Elle a une atmosphere tres sympathique comme une grande famille. Elle liera les amities entre nous, fera notre connaisances avec tous les autres ami(e)s novelles, transmettra les messages entre Ambassade Chinois en Suisse et notre membres. Elle aussi facilitera tous notre communications e.g. echanges d'informations,aides avec tous notre activities traditonelles,culturels,sociales,sportiques,academiques,physiques,politiques,etc en inclut. Elle toujours prendra les interets des notre membres si possible.

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Yue Zhang 's online developed/adapted protocols}

Applied biology: ? Aquaculture, fish endocrinology and artificial propagation. ? Fish population polymorphism genetics

Protein Chemistry: ? Bacterial protein expression: pET system etc ? Protein simple modification ? Western blotting

Bio-Rad system Western

Invitrogen LiCOR system Western

LiCOR system Western

and Protein co-immunoprecipitation (co-IP) assay

Protein co-immunoprecipitation (co-IP)

High throughput Protein co-immunoprecipitation (co-IP)

? In vitro Protein ?DNA binding assays (classic EMSA )

Clontech protein DNA binding assay

? In vitro GST pull ?down assays

Classical and modified Tandem affinity purification( TAP)

& Protein ID by Mass spectrometry

Chromatin immunoprecipitation

ChIP-tiling array assay ( Nimblegen and Affymetrix array) and ChIP- cloning /seq Molecular Biology:

BAC/fosmid DNA Recombineering

Mammalian lentivirus SiRNA

RNA interference C.elegans

Molecular cloning and sub-cloning; chemical competent cell

Molecular cloning and sub-cloning; ligation

? worm Mutagenesis (EMS/ENU) Knock-out and snp & FLP mapping ? Northern blotting ,Southern blotting

PCR/RT- PCR and real time qPCR, qRT-PCR

? Yeast two-hybrid assay,

LNA microRNA

? Promega luciferase reporter assay and miRNA sensor ? Affymetrix gene chip assay, BD Suppression Subtractive Hybridization(SSH)

BD Suppression Subtractive Hybridization(SSH)

? Computational analysis of DNA and protein sequences ? 3-D modeling (SwissModel) ? CGH, DNA methylation assays (Roche Nimblegen arrays). Cell Biology: ? Karyotype analysis and staining of plant tissue section ? Epifluorescence and Nomarski (DIC) Microscopy ( Zeiss, Olympus & Leica)

Immunofluorescence staining immunohistochemistry C.elegans

Immunofluorescence staining immunohistochemistry mammalian

? Confocal laser microscopy ( Olympus) ? Electron microscopic imaging

Gene gun bombardment

or microinjection

Cell culture:transfection1

Principle of FACS/ Flow Cytometry

CSSA en Universiey de Fribourg ( CSSAF)

Bienvenue a registre comme une CSSA membre, SVP contactez:

Executive :	ZHANG Yue
Phone:	++41 26 300 8863
Fax:	++41 26 300 9741
L'address:	Institut de Zoologie, Chemin du Musee 10, CH-1700 Fribourg, Suisse
Currier:	yue.zhang@unifr.ch

LIVING IN SWITZERLAND

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