Mr.Kulachat Sae - Jang

Ph.D student in Medical Technology

4436692 MTMT/D

Group 17

Assignment one

Restriction map analysis

Webcutter 2.0 is bioinformation tools serve to restriction map analysis in molecular biology which known base sequence and need to cut DNA sequence for add in expression plasmid vector and gene expression or subclone of fracment that easy for found residue base sequence. In addition to found restriction enzyme what suitable to cut those DNA sequence

Webcutter 2.0!

Welcome to Webcutter 2.0! This new version of Webcutter is a complete rewrite. Along with cleaner and more maintainable code, I am pleased to introduce the following new features:

Rainbow cutters Highlight your favorite enzymes in color or boldface for easy at-a-glance identification Silent cutters Find sites which may be introduced by silent mutagenesis of your coding sequence Sequence uploads Input sequences directly intoWebcutter from a file on your hard drive without needing to cut-and-paste Degenerate sequences Analyze restriction maps of sequences containing ambiguous nucleotides like N, Y, and R. Circular sequences Choose whether to treat your sequence as linear or circular Enzyme info Click into the wealth of references and ordering information at New England BioLabs' REBASE, directly from your restriction map results

Step by step manual of webcutter 2.0

1.Open website http://www.firstmarket.com/cutter/cut2.html

2.Input your title for sequence and paste the DNA sequence into the box

ex;

3.Select the type of analysis you would like

ex;

4.Click Analyze sequence

5.Result will show restriction map and restriction enzymes what suitable for those DNA sequence and you can select them to experiment.

component of results in webcutter will show;

- Restriction map

- Table of enzyme name that consist of enzyme name, number of cuts, positions of site.