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COURSE OUTLINE

PART 1

A.        Mutation
            i.        point mutations

substitution, addition, deletion
reversions & suppressor mutations
transitions & transversions
            ii.       chromosomal mutations
duplication, inversion, deletion, translocation, reciprocal translocation
            iii.       genomic mutations
polyploidy, aneuploidy
            iv.       mutagens and their modes of action
nitrous acid, hydroxylamine, alkylating agents, heat, base analogues, acridines, ionising radiation, UV light


B.        DNA repair

photoreactivation, excision repair, mismatch repair, proofreading, O6-methylguanine methyltransferase, postreplication repair


C.        Isolation, selection and analysis of mutants
            i.        morphological and auxotrophic mutants
            ii.       replica plating, enrichment, toxic analogues
            iii.      drug resistance, lethal mutations (BascX, Clb in Drosophila)
            iv.      Cy/Pm; Di/Sb stock in Drosophila
            v.       complementation & complementation analysis

PART 2

A.        Gene and genome structure

            i.       concept of the gene

central dogma & exceptions
definition of the gene
            ii.      structure of the gene
differences between eukaryotic and eubacterial genes
promoters, leader, introns & exons, enhancers, trailers
            iii.     Organisation of the genome
the C-value paradox
classification of genomic DNA
features of eukaryotic genomes
genes, families, & superfamilies; pseudogenes; SINEs, LINES & transposons; satellite DNA; VNTRs
            iv.     Centromeres & telomeres (telomerase and telomere replication)

            v.      extranuclear genomes

detection of extrachromosomally-inherited traits
reciprocal crosses, heterokaryon formation, heteroplasmon formation, linkage analysis
chloroplast & mitochondrial DNA


B.        Gene expression

            i.        Transcription and translation

RNA polymerases
            ii.      RNA processing
capping, splicing, tailing
splicing of various introns - groups I, II, IV and pre-mRNA
            iii.    regulation of gene expression
transcription factors
lac & trp operons:  catabolite repression & attenuation


PART 3

A.        Genetic Mapping in bacteriophages

lysis and lysogeny
mixed infections
two- and three-point crosses


B.        Genetic mapping in bacteria
            i.        conjugation

F-, F+, Hfr, F', mapping by interrupted mating, merodiploids, sexduction
            ii.      transduction
Generalised and specialised transduction,
co-transduction, three-factor transduction
            iii.     transformation
cotransformation


C.        Genetic mapping in fungi
            i.        ordered tetrads

first and second division segregation
linkage in meiotic tetrads
            ii.        parasexual analysis
heterokaryon formation
determination of linkage relationships
PART 4

A.           Recombination

generalized recombination, the Holliday model; Meselson-Radding & Szostak variations
B.       Transposons and TranspositionAdded 11/26/2000

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