Log of Observations-Phase One
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01/02/01 (Updated by Emily)-Today was the start of our project. Until this point, we have attempted to gather information about the procedure that we need to follow, but alas not much information is available on the exact procedures. At this point all we can do is deduce and experiment like pioneers. We have decided on the concentrations for our antibiotics in relation to the sensitivity disks as follows: 01/03/01(Updated by Emily)-After the concentraions were determined we impregnated the disks by soaking them in the solutions, and then placed the disks on the surface of the previously inoculated agar. In addition to the two plates of bacteria set up for a control, we had a plate of each antibiotic per bacterial species. These plates were placed in the incubator at the end of the transfer. We have completed all of the needed transfers and are ready for observation. At this point in time, our hypothesis is that the higher concentration antibiotics will have a greater effect on the bacteria. However, tomorrow shall prove more. 
01/04/01(Updated by Emily)-Upon observing our plates, we were not able to see anything. There was an even distribution of bacterial growth, and there were no zones of inhibition at all. Due to this, we attempted to redo one set of plates using the antibiotic Oxacillin. Hopefully tomorrow will produce a result.  |
Second Concentration- 1/100 g in 4 ml distilled water