(Ume) Matsuzaka Sensei
Matsuzaka and Yoshinaga-sensei are a pair of female teachers at Shin-chan's kindergarten. Like gas and fire, the mixture of the two is much more interesting than either one alone. The two teachers have an ongoing rivalry and are always trying to upstage each other. Periodically, the rivalry will flame out of control and of course, Shin-chan is probably somewhere nearby fanning the flames.
Matsuzaka-sensei can be identified by her long black hair and the somewhat sinister aura about her. However, even though she won't admit it, she cares for Yoshinaga like a sister.
Matsuzaka-sensei is also 24 years old and she's quite aware of the fact that she isn't getting any younger. Because of this, one of her biggest priorities in life is entrapping some successful young man in marriage but Shin-chan always seems to be around at exactly the wrong time.

(Midori) Yoshinaga Sensei
Yoshinaga is a bouncy and enthusiastic teacher. However, her playful disposition is no challenge for Shin-chan who happens to be in the class that she teaches. But to her credit, she hasn't killed him yet. Most normal people without Yoshinaga's resiliency would have made sure Shin-chan ended up in a shallow grave a long time ago.
Yoshinaga-sensei has brown hair which is tied back with a ribbon. She also claims 89cm.

"Yakuza ja nai!" Enchiyou Sensei
It's baffling trying to figure out how some people chose the professions that they did. Enchiyou-sensei is the principal at Shin-chan's kindergarten. He's a big, hulking man who always wears sunglasses and the same tacky suit, has a mustache and a scar on his face.. the kind of look that just shouts, "Japanese mobster." However, he seems to have both of his pinkies (Japanese mobster often lack them) and his suit jacket hides any possible body tattoos but it wouldn't be a far stretch of the imagination. In fact, he has been mistaken for a gangster on several different occasions. In reality, he's a gentle person and doesn't like it when Shin-chan teases him about looking like a gangster. Maybe in a future episode, he'll personally show Shin-chan how to make concrete shoes.