MOLECULAR MODELLING AND STRUCTURAL  ANALYSIS OF PAPAIN 

   

               

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Introduction

Secondary Structure

Hydrophobicity of Papain

Alignment of Papain
against A. thaliana

Papain compared to A. thaliana protein

Homology Modeling

Animated  Models

Graphs and Spreadsheet



Alignment of  Papain(Accession P00784)  against AT4g35350 [Arabidopsis thalia]

                                                                     
 
       The result shows a significant similarity between papain and cysteine proteinase(A. thaliana) (1)



                                                        ANTHEPROT DOT PLOT
This is a bidimensional plot in which the sequence of the protein to be compared is plotted at the X-axis, and the sequence of the protein we want to compare with is plotted at the Y-axis. Using an amino acid substitution matrix, it is calculated a degree of similarity between the two proteins. If the degree of similarity is significant, a dot is plotted in the diagonal of the graph (3).



The dot plot matrix reveal significant similarities between Papain(pdb 1cvz) and cysteine proteinase obtained from A . thaliana


                    3D ALIGNMENT OF PAPAIN AGAINST A. thaliana PROTEIN( CAA18734)                                                                      

 Fig. 1. 3D homology alignment of papain(1cvz) chain A, against cysteine proteinase of
A. thaliana.  Papain(pdb1cvz) is colored purple, while blue represents A. thaliana protein(CAA18734)  (12)

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